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Asexual Propagation and Seedling Cultivation Method for Schisandra chinensis: A Detailed Explanation of Tissue Culture Technology

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As an emerging technology and research tool, plant tissue culture is flourishing in all fields of plant science and has been widely applied in production sectors such as agriculture, forestry, horticulture, and Chinese herbal medicine, demonstrating enormous advantages. It provides large numbers of seedlings in a short time to meet production needs. As a means of asexual propagation, tissue culture can propagate 60,000 shoots from 1 apple bud in 8 months, 1 million seedlings from 1 poplar axillary bud in 1 year, and 500,000 seedlings from 1 Schisandra chinensis bud in 1 year, thus

As an emerging technology and research tool, plant tissue culture is flourishing in all fields of plant science and has been widely applied in production sectors such as agriculture, forestry, horticulture, and Chinese herbal medicine, demonstrating enormous advantages. It provides large numbers of seedlings in a short time to meet production needs. As a means of asexual propagation, tissue culture can propagate 60,000 shoots from 1 apple bud in 8 months, 1 million seedlings from 1 poplar axillary bud in 1 year, and 500,000 seedlings from 1 Schisandra chinensis bud in 1 year, which shows that its application prospects are very broad.

(1) Obtaining and treating explants: Take axillary buds of a Schisandra chinensis variety or superior line as tissue culture material. Cut 2 cm-long young stem segments with axillary buds, rinse with water for 10 minutes, sterilize with 2% sodium hypochlorite for 20 minutes, rinse 5 times with sterile water, then blot dry with sterile gauze, and inoculate onto the medium for inducing axillary bud germination through aseptic operation.

(2) Bud induction: The induction medium uses MS + 6-BA 4 mg/L + IBA 0.2 mg/L + LH 1000 mg/L; MS vitamins doubled, sucrose 30 g/L, agar 9 g/L, pH 5.8, maintain temperature at 18-20°C, light for 10 hours, light intensity 800-1200 lux, and buds can grow after 4-5 weeks.

(3) Subculture: Cut buds that have grown to 2 cm from the branches and inoculate them onto subculture medium for cultivation. The medium is MS + 6-BA 4 mg/L + IBA 0.2 mg/L + LH 1000 mg/L; MS vitamins doubled, sucrose 30 g/L, agar 9 g/L, pH 5.8. Culture conditions are temperature 18-20°C, light for 10 hours, light intensity 800-1200 lux. After 4 weeks, bud clusters can grow. Cut off the buds and continue to expand propagation until the required quantity is reached.

(4) Rooting culture: Transfer bud cluster shoots larger than 2 cm onto rooting medium. The rooting medium is: NH4NO3 825 mg/L, KNO3 950 mg/L, MgSO4·7H2O 195 mg/L, KH2PO4 100 mg/L, Fe-EDTA 35 mg/L, KI 0.83 mg/L, H3BO3 6.2 mg/L, MnSO4·4H2O 0.25 mg/L, CuSO4·5H2O 0.025 mg/L, CoCl2·6H2O 0.025 mg/L, inositol 200 mg/L, nicotinic acid 1.0 mg/L, pyridoxine 1.0 mg/L, thiamine 0.2 mg/L, hydrolyzed albumin 1000 mg/L, indolebutyric acid 2 mg/L, sucrose 15 g/L, agar 9 g/L. Environmental conditions: temperature 20-25°C, light for 14 hours, light intensity 2000 lux, and rooting can occur after 4-6 weeks.

(5) Hardening: Wash the medium from the roots of seedlings that have grown several roots over 1 cm long and have fibrous roots, and plant them in nutrition pots with a diameter of 8 cm. The growing medium in the nutrition pots is disinfected humus soil. Maintain relative humidity at 85%, temperature 20-25°C, light at 2000-2800 lux. After 1 week, the relative humidity can be gradually reduced to natural conditions. After 4-5 weeks, when the seedlings grow new leaves and new roots, they can be transplanted, with a survival rate above 90%.

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