Cultivation Methods for Lingzhi (Ganoderma lucidum) and Control Techniques for Neurospora

1. Nutrient management: Before planting lingzhi, we need to prepare sawdust and wheat bran, as well as the necessary fertilizer. We mix the sawdust, wheat bran, and urea evenly, let it sit for a few hours, and then it is ready for filling. Of course, the wheat bran can be replaced with rice straw powder or corn stalk residue, and the urea can also be replaced with ammonia fertilizer; the results are all very good. You can prepare several kinds of nutrients. Nutrients are the source of nutrition for lingzhi, so nutrients are very important.

2. Filling management: After the nutrients are prepared, we can use ordinary plastic bags for filling. Each bag holds about 200 to 300 grams. When filling the bags, pay attention to making them firm and the surface smooth, then poke a ventilation hole in the middle, seal the opening with a cotton plug, and wrap a layer of kraft paper on the outside.

⟧ 3. Sterilization management: Because the nutrients during preparation, as well as the bags and bottles used for filling, may contain a large number of bacteria, we need to carry out sterilization treatment to ensure the healthy growth of lingzhi. Use high-pressure sterilization, so that inoculation can be carried out the next day. If we use atmospheric-pressure sterilization, inoculation must be carried out immediately after sterilization, otherwise bacteria will invade.

4. Inoculation management: The environmental requirements during inoculation are very strict. It is best to have a dedicated inoculation room. When entering the inoculation room, the tools used for inoculation need to be disinfected. Next, the inoculation personnel need to disinfect themselves, then use tools to take out the cultured spawn and put it into the bags or bottles filled with nutrients, then seal the bags or bottles well and place them in a culture room at about 25 to 28 degrees for cultivation. After about one month, mushroom primordia will grow, and then the temperature needs to be raised by about one more degree to continue growth. When the surroundings become hard, they can be harvested.

How to control Neurospora in lingzhi? Please introduce the harm of Neurospora in lingzhi:

  Neurospora in lingzhi is also called Neurospora sitophila or red bread mold, bread mold, Neurospora, and Monilia sitophila. It belongs to the subdivision Ascomycotina, order Sordariales, genus Neurospora. It is a contaminant fungus that poses a great threat in the spawn production of lingzhi edible fungi. It spreads relatively quickly.

  Occurrence characteristics of Neurospora in lingzhi: This pathogen is widely distributed and lives on organic matter. It spreads through airflow. Incomplete disinfection of the culture material, incomplete disinfection and sterilization of the inoculation room or inoculation box, failure to follow aseptic operating procedures during inoculation, failure to replace cotton plugs after they become damp, damage or cracks in spawn bags, or contamination of the culture material by contaminants carried in the stock spawn or mother spawn used for inoculation can all lead to infection.
Neurospora prefers high temperature and high humidity, and grows rapidly when oxygen is sufficient. High air humidity and poor ventilation in the spawn culture room are conducive to the occurrence and spread of the disease. The occurrence of the pathogen is closely related to the indoor and outdoor environmental sanitation conditions of the spawn production room.

  Control methods for Neurospora in lingzhi:

  (1) Do a good job of environmental sanitation in the spawn production room, centralize the treatment of waste and bury it deeply, and prevent the growth and spread of the pathogen.

  (2) Thoroughly sterilize the culture material, avoid dampening the cotton plugs, and reduce damage to the spawn bags.

  (3) Disinfect the inoculation site and tools with potassium permanganate.

  (4) During inoculation, replace the cotton plugs in time, and sprinkle a layer of lime powder on the cotton plugs to absorb moisture and prevent bacteria.

  Commonly used agent: potassium permanganate.
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