What are the key points to note when growing lingzhi (Ganoderma lucidum)?
What are the key things to note when growing lingzhi? Here is an introduction. There are 4 points to note when growing lingzhi, listed in detail below for reference: 1. Preparation of lingzhi growing substrate: ① about 75% mixed sawdust, about 25% wheat bran, about 0.2% ammonium sulfate, water content of the mixed substrate 70%; ② about 75% mixed sawdust...
What are the key things to note when growing lingzhi? Here is an introductionThere are 4 points to note when growing lingzhi, listed in detail below for reference:
1. Preparation of lingzhi growing substrate:
① about 75% mixed sawdust, about 25% wheat bran, about 0.2% ammonium sulfate, water content of the mixed substrate 70%;
② about 75% mixed sawdust, about 25% wheat bran, water content of the mixed substrate 60%;
③ about 50% mixed sawdust, about 50% wheat bran, 0.1% urea, water content of the mixed substrate 60%;
④ 79% cottonseed hulls, about 20% wheat bran, 1% sucrose, water content of the mixed substrate 60%?65%;
⑤ 50% corn cob residue, about 30% mixed sawdust, about 20% wheat bran, water content of the mixed substrate 60%?65%;
⑥ 75% poplar leaves, 25% rice bran (or wheat bran), water content of the mixed substrate 60%;
⑦ 45% rice straw powder, about 30% mixed sawdust, about 25% wheat bran (or rice bran), water content of the mixed substrate 60%?65%.
2. Filling and sterilization of the lingzhi growing substrate:
After mixing the growing substrate, let it sit covered for half an hour before filling. A standard plastic bag can hold 0.25-0.3 kg of dry substrate. When filling the bag, press it down firmly by hand, and keep the substrate surface level; then use a conical wooden stick to make an air hole 2.5 cm in diameter from the center of the substrate surface, plug the bag opening well with a cotton plug, and wrap it with a layer of kraft paper. If filling bottles, use wide-mouth bottles or mushroom bottles with a volume of 500-1000 ml and a mouth diameter of 3.3-4.6 cm; the filling method is basically the same as the bag method.
After filling, arrange the substrate bags (or bottles) in layers in the pot, and sterilize under a pressure of 1.5 kg/sq cm for 1-2 hours, or sterilize at normal pressure for 8-10 hours. It is required to fill, sterilize and inoculate on the same day.
3. Inoculation and culturing of lingzhi:
Inoculation is best done in an inoculation box; if there is no inoculation box, it can also be done above the flame of an alcohol lamp or above boiling water steam.
The inoculation room and inoculation tools should also be strictly disinfected. The worker should wear a mask and wash hands with soap three times. Use an inoculation rake or tweezers to take out a piece of spawn the size of a jujube from the bottle, quickly put it into the cultivation substrate bottle (bag), then plug the bottle opening (or bag opening) well with a cotton plug and wrap it with kraft paper, and move it to a sterilized culture room for culturing and sterilization.
Keep the room temperature at 24°C--28°C; about 25 days after inoculation, the mycelium will have grown throughout the bottle (bag).
During the mycelial growth stage, management should be strengthened to prevent contamination by foreign microorganisms; contaminated ones should be discarded promptly. When the bottle (bag) is fully covered with mycelium, white primordia the size of a fingertip, namely the fruiting body primordia, gradually appear on the surface of the growing substrate.
When their growth approaches the cotton plug, the cotton plug can be removed, the room temperature controlled at 26°C--28°C, the relative humidity raised to 80%--90%, scattered light provided, and ventilation carried out every day; after about 20 days, the stipe can grow out of the bottle (bag) opening, and the cap differentiates at the end of the stipe. When the pale white or pale yellow color at the edge of the cap disappears, the edge of the cap stops growing and hardens, and when the color changes from bright to dark pinkish brown, it can be harvested.
4. Methods for disease control in lingzhi:
The inoculation process must be strictly aseptic; the growing substrate must be thoroughly sterilized; proper ventilation and reduced humidity; for mild infection, use a sterilized blade to scrape off the local foreign microorganisms and the surrounding bark, then apply thick lime milk, or stuff absorbent cotton dipped in 75% alcohol into the holes; those with severe contamination should be discarded promptly.
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