-
Where to buy edible mushroom spawn? Detailed explanation of the mother culture inoculation method
Where can you find edible mushroom spawn, and how do you inoculate the mother culture
-
Four Simple Preservation Methods for Edible Mushroom Mother Cultures and Cultivation Techniques
Four Simple Preservation Methods for Edible Mushroom Mother Cultures
-
Essential Knowledge for Introducing Edible Fungi and Simple Methods for Preserving Mother Cultures
Simple Preservation of Edible Fungus Mother Cultures
-
How to Identify Mother Cultures and Spawn of Edible Fungi? A Detailed Guide to the Methods
Shiitake (Lentinula edodes) spawn: the mycelium is snow-white, thick and strong, spread out, and uniform, with even margins and a fast growth rate; on the bottle wall it develops in a fan-shaped, feather-like pattern, growing vigorously, and secretes soy-sauce-colored droplets. After the mycelium has fully covered the bottle, within 10 to 15 days an amber-colored transparent liquid is commonly seen, along with the distinctive fragrant aroma of shiitake, with no foul or musty odor. If the mycelial column inside the bottle pulls away from the bottle wall, this indicates that the substrate moisture is too high; if it has pulled away and the mycelial column also begins to shrink, with the surface mycelial skin thickening and turning dark brown, this indicates that the spawn has begun to age and cannot
-
Detailed Guide to Making Mother Culture Media for Edible Mushrooms
1. 800 g corn, 20 g glucose, 20 g agar, 1.5 L rice washing water. First cook the corn kernels in dilute rice washing water until a small number of kernels burst, then scoop them out and filter. Take the filtrate and continue heating (if the filtrate is less than 1 L, add clean water to make up the volume), add the agar and glucose, stir until the agar is completely dissolved, then dispense into test tubes, and after sterilization take them out and slant them to set. 2. 200 g fresh oyster mushroom or mushroom stipes, 20 g glucose, 20 g agar, 1 L water. The preparation method is the same as for potato dextrose agar medium. 3. Bran 2
-
Detailed Explanation of a Simple Method for Making Mushroom Mother Cultures
For making mushroom mother cultures, PDA or PSA and their improved media are mostly used, but in some remote areas or for small-scale production, the raw materials in the formula are relatively expensive (generally analytical grade) and are not easy to buy in rural areas, delaying production. Here are several simple methods. 1. Gelatin-type medium. Gelatin refers to edible gelatin, generally sold in food and chemical shops, and the price is relatively low. Gelatin used in food production as a coagulant or solidifying agent can replace agar (bacteriological agar, agar-agar) as a coagulant in mother culture production. The author conducted experiments in the early 1990s and used it in production, proving the effect is good, but the purchased product needs to be tested for coagulating properties. After finally determining the amount to use per 1000 mm, it is necessary to keep using that brand and not change it at will. The general production formula is: 200 grams of potato, 30 grams of wheat bran, 20 grams of white sugar, about 50 grams of gelatin, and 1000 milliliters of water. 2. Raw grain-type medium. That is, grain medium. @#@245@#@, corn, beans, etc. can all be used as mother culture media, and the production effect is very good. The method is: corn and soybeans need to be cracked so that their size is similar to wheat grains, soaked until there is no white core, boiled with boiling water, then quickly rinsed with cold water, taken out and immediately mixed with an appropriate amount of wheat bran, and then put into test tubes. When using this medium, it is recommended to switch to large test tubes; generally 20×200 or 25×200 specifications can be used, which is convenient for operation and correspondingly increases the quantity produced at one time, and is cost-effective in both economic and time terms. 3. Potato-type medium: choose large, smooth potatoes, peel them, and cut them into wedge-shaped strips. The potato strips are 12-15 cm long, with the two ends respectively 13 cm and 0.2 cm thick, and the width is determined according to the diameter of the test tube, generally below 2 cm, and they should be made as semicircular as possible so that the rounded edge is close to the test tube wall. Put them into test tubes and carry out routine sterilization and other operations. 4. Pumpkin strip medium: after pumpkins mature in autumn, pumpkin strips can be directly cut as a mother culture medium by referring to the potato-type medium, and the preparation is the same as above. 5. Apple puree medium: peel and core ripe apples, grind them into puree, put them into test tubes, and sterilize routinely. The only thing is that when inoculation is needed, use a sterilized wooden stick to press the puree into a long strip shape, making it similar to ordinary medium, and then carry out routine inoculation. 6. Carrot strip medium: this can be done by referring to the potato medium.
-
How to identify edible mushroom mother cultures by appearance?
Excellent purified mushroom cultures are the fundamental guarantee for successful edible mushroom cultivation. The appearance identification methods for several edible mushroom mother cultures are introduced as follows: Shiitake mushroom mycelium on potato dextrose agar (PDA) medium is pure white, thick and robust, appearing floccose or velvety; after filling the test tube it has a certain ability to climb the wall, with edges showing irregular wavy curves. At around 24°C, the mycelium generally fills the test tube slant in 12-15 days. It has a distinctive shiitake aroma. Oyster mushroom mycelium on PDA medium is snowy white, dense and thick
-
What are the precautions for buying mushroom mother cultures?
Through surveys in Pulandian and the surrounding areas, it was found that in actual production most mushroom growers do not buy spawn for direct cultivation, but instead buy mother cultures and propagate the spawn themselves. Although this can save costs, the following points should be noted when purchasing to avoid unnecessary losses. 1. Ask about culture age. When buying mother cultures, first ask about the culture age, which directly affects the quality and finished-product rate of the spawn. 2. Ask about the substrate. Mother cultures grown on different substrates look different; for example, grain-based cultures have denser and whiter mycelium than straw-based cultures, and straw-based cultures with more wheat bran, soybean powder, cornmeal, etc. grow more vigorously than those with less of these supplements. Do not let the nutritional richness or poverty of the medium affect your judgment of culture quality. Judge culture quality according to the different substrates. In addition, relatively nutrient-rich substrates age quickly after the culture finishes growing and should not be stored too long.
条件筛选