Detailed Explanation of a Simple Method for Making Mushroom Mother Cultures
Mushroom Cultivation0 views
For making mushroom mother cultures, PDA or PSA and their improved media are mostly used, but in some remote areas or for small-scale production, the raw materials in the formula are relatively expensive (generally analytical grade) and are not easy to buy in rural areas, delaying production. Here are several simple methods. 1. Gelatin-type medium. Gelatin refers to edible gelatin, generally sold in food and chemical shops, and the price is relatively low. Gelatin used in food production as a coagulant or solidifying agent can replace agar (bacteriological agar, agar-agar) as a coagulant in mother culture production. The author conducted experiments in the early 1990s and used it in production, proving the effect is good, but the purchased product needs to be tested for coagulating properties. After finally determining the amount to use per 1000 mm, it is necessary to keep using that brand and not change it at will. The general production formula is: 200 grams of potato, 30 grams of wheat bran, 20 grams of white sugar, about 50 grams of gelatin, and 1000 milliliters of water. 2. Raw grain-type medium. That is, grain medium. @#@245@#@, corn, beans, etc. can all be used as mother culture media, and the production effect is very good. The method is: corn and soybeans need to be cracked so that their size is similar to wheat grains, soaked until there is no white core, boiled with boiling water, then quickly rinsed with cold water, taken out and immediately mixed with an appropriate amount of wheat bran, and then put into test tubes. When using this medium, it is recommended to switch to large test tubes; generally 20×200 or 25×200 specifications can be used, which is convenient for operation and correspondingly increases the quantity produced at one time, and is cost-effective in both economic and time terms. 3. Potato-type medium: choose large, smooth potatoes, peel them, and cut them into wedge-shaped strips. The potato strips are 12-15 cm long, with the two ends respectively 13 cm and 0.2 cm thick, and the width is determined according to the diameter of the test tube, generally below 2 cm, and they should be made as semicircular as possible so that the rounded edge is close to the test tube wall. Put them into test tubes and carry out routine sterilization and other operations. 4. Pumpkin strip medium: after pumpkins mature in autumn, pumpkin strips can be directly cut as a mother culture medium by referring to the potato-type medium, and the preparation is the same as above. 5. Apple puree medium: peel and core ripe apples, grind them into puree, put them into test tubes, and sterilize routinely. The only thing is that when inoculation is needed, use a sterilized wooden stick to press the puree into a long strip shape, making it similar to ordinary medium, and then carry out routine inoculation. 6. Carrot strip medium: this can be done by referring to the potato medium.
For the production of mushroom mother cultures, PDA or PSA and their improved media are mostly used, but in some remote areas or for small-scale production, the raw materials in the formula are relatively expensive (generally analytical grade) and not easy to buy in rural areas, which delays production. Here are several simple methods. 1. Gelatin-type medium. Gelatin refers to food-grade gelatin, generally available in food and chemical shops at a relatively low price. Gelatin, used as a gelling agent or solidifying agent in food production, can replace agar (agar-agar, agar) as a gelling agent in mother culture production. The author conducted experiments in the early 1990s and used it in production, proving the effect is quite good, but the purchased product needs a gelling test, and after finally determining the amount to use per 1000 mm, one must stick to that brand and not change it at will. The general production formula is: potato 200 g, wheat bran 30 g, white sugar 20 g, gelatin about 50 g, water 1000 ml. 2. Raw grain-type medium. That is, grain medium. @#@245@#@, corn, beans, etc. can all be used as mother culture media, and the production effect is very good. The method is: corn and soybeans need to be cracked so that their size is similar to wheat grains, soaked until there is no white core, boiled in boiling water and then quickly rinsed with cold water, and after being taken out, an appropriate amount of wheat bran is immediately mixed in, then put into test tubes. When using this medium, it is recommended to switch to large test tubes; generally 20×200 or 25×200 specification test tubes can be used, which is convenient for operation and correspondingly increases the one-time production quantity, and is economical in both cost and time. 3. Potato-type medium: choose large, smooth potatoes, peel them and cut them into strip pieces; the potato strips are 12-15 cm long, with the two ends respectively 13 cm and 0.2 cm thick, and the width is determined according to the test tube diameter, generally below 2 cm, and try to make them semicircular so that the rounded edge fits close to the test tube wall. Put them into test tubes and carry out routine sterilization and other operations. 4. Pumpkin strip medium: after pumpkins mature in autumn, pumpkin strips can be cut directly as mother culture medium by referring to the potato-type medium, with the same preparation as above. 5. Apple puree medium: peel and core ripe apples, grind them into puree, put them into test tubes, and sterilize routinely. The only thing is that when inoculation is needed, use a sterilized wooden stick to press the puree into a long strip shape, making it similar to ordinary medium, and then carry out routine inoculation. 6. Carrot strip medium: it can be prepared by referring to the potato medium.