Detailed Explanation of Edible Mushroom Spawn Production Techniques and Methods

First, produce high-quality mother spawn, and at the same time prepare the mother spawn culture medium. Then place the mother spawn in the incubation room. After the mycelium grows over the slanted surface of the culture medium, it can be used to inoculate the stock spawn. Next, produce the stock spawn, and at the same time prepare the culture medium. When the culture medium has cooled to room temperature, carry out inoculation. Generally, after 20-25 days it can fill the bottles. Finally, produce the planting spawn. After it fills the bottle walls, it can be sown.

1. Mother spawn culture medium formula: potato 200 g, glucose 20 g, agar ZO g, potassium dihydrogen phosphate 1 g, magnesium sulfate 0.50 g, water 1000 ml, pH 5.50-6.50.

Inoculation and culture: After inoculation by the conventional method, place the mother spawn in the incubation room at a temperature of 23-25°C and an air relative humidity of about 65% and culture for 7-10 days. When the mycelium grows over the slanted surface of the culture medium, it can be used to inoculate the stock spawn.

2. Stock spawn formula: wheat (corn): 95%; gypsum powder: 2O% superphosphate: 2O%; urea: 0.50%; white sugar: 0.50%; pH: 5.50-6.50. 2.1.2 Seed hull: 87%; bran: 10%; sucrose white sugar: 0.50%; gypsum powder: 1%; superphosphate: 1%; urea: 0.50%; add water 120%-130%.

Inoculation and culture: When the culture medium for strain A is taken out of the pot and cooled to room temperature, carry out inoculation. Each test tube can inoculate 5-6 bottles of stock spawn. The culture conditions are the same as for the mother spawn. Generally, after 20-25 days it can fill the bottles. Note: When culturing in wine bottles, shake the bottles at 10-12 days, then continue culturing. The characteristics of high-quality stock spawn are snow-white, neat mycelium, mycelium covering the bottle walls and surface, and a mushroom aroma.

3. Production of planting spawn

Inoculation and culture: First, rub the hands and disinfect the outside of the stock spawn bottles with 75% alcohol or 3% Lysol, then use a sterilized inoculation shovel (hoe* to loosen the stock spawn, and above the flame of an alcohol lamp inside the inoculation box (on a clean bench) pour a small amount of stock spawn into the sterilized bottle material. Each bottle of stock spawn inoculates 20-25 bottles. The culture conditions are the same as for the stock spawn. Generally, it can fill the bottles in 20-25 days. After it is full, it should be sown immediately. If not used temporarily, it can be stored dry at 10-14°C for no more than 10 days; at 2-4°C, storage should not exceed 20 days. For planting spawn preserved at low temperature, restore it at room temperature for 1-2 days before use.