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Detailed Explanation of Methods for Storage and Preservation of Tea Plant Vegetative Propagules

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Direct field preservation of tea plant vegetative propagules requires a large amount of land and labor, so long-term preservation techniques for tea roots, seedlings, seeds and pollen have been studied. Root preservation involves sterilizing the lignified roots before spring sprouting, storing them together with sterilized semi-dry soil or sphagnum moss in plastic bags, and placing them in an environment of 1-5°C. After 7 years of storage, the occurrence of adventitious buds can reach up to 70%. Stored at 1°C for 6 years, the roots can still regenerate. Under low-temperature storage conditions, the consumption of carbohydrates in root tissues

Direct field preservation of tea plant vegetative propagules requires a large amount of land and labor, so long-term preservation techniques for tea roots, seedlings, seeds and pollen have been studied.

Root preservation involves sterilizing the lignified roots before spring sprouting, storing them together with sterilized semi-dry soil or sphagnum moss in plastic bags, and placing them in an environment of 1-5°C. After 7 years of storage, the occurrence of adventitious buds can reach up to 70%. Stored at 1°C for 6 years, the roots can still regenerate. Under low-temperature storage conditions, the consumption of carbohydrates in root tissues can be sustained for about 12 years. Root survival can be judged by the amount of potassium leached from the roots.

For seedling preservation, 2-year-old cutting seedlings can be removed in early spring and stored sealed at 5°C for 4 years, with survival rates of different cultivars ranging from 20% to 26%. The stored tea seedlings, after being taken out and planted, can be used as hybridization material the following year, with a fruit-setting rate of 63% to 70%. The maximum storage period can reach 9 years.

After pollen is stored at -80°C for 5 years, it still has a germination rate of 70%, and after 12 years the germination rate is 60%, which makes it possible to hybridize cultivars with different flowering periods.

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