Methods and Key Techniques for Preserving Edible Mushroom Strains

Two methods are usually used for preservation: the slant low-temperature preservation method. This is the simplest preservation method, in which vigorously growing, uncontaminated slant tubes are placed in a refrigerator and preserved at a low temperature of 4°C~6°C, after which they are subcultured onto fresh slant medium every 2~3 months and, after incubation, preserved again. The other is the liquid paraffin preservation method. In this method, sterilized liquid paraffin is injected in a sterile room into the slant tubes to be preserved, with the paraffin added to a level 1 cm above the slant. Then a wooden stopper or rubber stopper is inserted, the opening is sealed with wax, and the tube is placed upright in a dry place indoors, where it can be preserved for more than one year.