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Methods and Technical Key Points for Preserving Edible Mushroom Spawn

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1. Distilled water method. Suitable for the spawn of oyster mushroom, enoki mushroom, and shiitake mushroom. 1. Preserving oyster mushroom and enoki mushroom spawn. First, move the test-tube spawn that is at the eight-tenths mature stage and awaiting preservation into an inoculation box, and after sterilization, use a medical syringe to inject distilled water through the test-tube stopper until the entire surface of the culture medium is submerged, up to 2 cm from the tube mouth. Seal the tube mouth with mineral wax, and at normal temperature place it upright in a cool, shady place, where it can be preserved for 1-1.5 years. 2. Preserving shiitake mushroom and wood ear mushroom spawn. Use a 150 x 15 mm finger-shaped tube to place solid or

I. Distilled water method. Suitable for the spawn of oyster mushroom, enoki mushroom, and shiitake mushroom.

1. Preserving oyster mushroom and enoki mushroom spawn. First, move the test-tube spawn that is at the eight-tenths mature stage and awaiting preservation into an inoculation box, and after sterilization, use a medical syringe to inject distilled water through the test-tube stopper until the entire surface of the culture medium is submerged, up to 2 cm from the tube mouth. Seal the tube mouth with mineral wax, and at normal temperature place it upright in a cool, shady place, where it can be preserved for 1-1.5 years.

2. Preserving shiitake mushroom and wood ear mushroom spawn. Using a 150 x 15 mm finger-shaped tube, under aseptic operation in an inoculation box, place solid or semi-solid test-tube spawn cultured to the seven-tenths mature stage into a 25 x 200 mm large test tube, inject distilled water into the large test tube up to 1 cm from the tube mouth, wrap the opening with a rubber stopper, reinforce and seal the outside with mineral wax, and place in a cool, shady place. Wood ear mushroom spawn can be preserved for 7-8 months, and shiitake mushroom spawn can be preserved for 9-12 months.

This method can also preserve other spawn at natural temperature.

II. Urea temporary storage method. Place bagged urea in a dark, dry, well-ventilated place, bury the main body of the test-tube spawn in the urea granules with the test tube mouth exposed, and the spawn vitality can be preserved for as long as 30 days. This method can be carried out under a constant low-temperature environment.

III. Liquid paraffin preservation method. Select chemical-grade liquid paraffin. After autoclaving, place it in a 40 degrees C constant-temperature incubator for several hours or in a desiccator for several days, then use a sterile pipette or syringe to draw liquid paraffin and inject it into the spawn test tube. The injection amount should be about 1 cm above the tip of the slant, and the tube mouth should be plugged with a cotton plug and sealed well with paraffin. Standing upright in a clean, dry, cool, low-temperature place, it can be preserved for 5 years.

IV. Wheat grain preservation method. Take high-quality @#@245@#@, wash it, soak it in 20 degrees C water for 5 hours, let it dry slightly, and then put it into tubes. The filling amount should preferably be 1/4-1/3 of the tube length. After sterilizing the test tubes filled with wheat grains, shake them loose while hot. After cooling, inoculate with mycelium. Culture at a suitable temperature until sparse mycelium appears on most wheat grains, then terminate culturing. The spawn can be placed in a dry place below 25 degrees C and preserved for 1-2 years.

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