How Do You Assess the Quality of Edible Mushroom Spawn? Practical Methods to Help You Tell Good from Poor
The quality of edible mushroom spawn directly affects the success or failure of production and the level of yield. Therefore, assessing the quality of edible mushroom spawn is crucial for growers.
1. Direct visual observation and assessment
In appearance, if the spawn mycelium is dense white, thick and strong, and elastic, it has strong vitality; if the spawn mycelium has shrunk, is dry and lacking in color, or the mycelium has autolyzed and produced a large amount of reddish-brown liquid, then its vitality has already weakened and it should no longer be used; if wood-block spawn remains hard and solid, it belongs to spawn with strong vitality, but if the wood blocks have become softened and loose, then it has aged and is not suitable for use.
2. Cultivation observation and assessment
For spawn that has been isolated, bred or introduced, observe through cultivation the adaptive characteristics of the mycelium with respect to dryness, moisture and temperature. For example, place the mycelium under relatively dry, relatively moist, and moderately dry-moist conditions for cultivation. If the mycelium grows well under the first two conditions, and grows best under the moderately dry-moist condition, then it shows that it is good spawn.
3. Liquid culture assessment
Prepare a 2% sugar water solution and sterilize it by conventional means. Pick out a spawn block the size of a soybean and place it into 100 ml of the above solution, then culture it at 25-28°C for 3-7 days. If bubbles appear on the liquid surface, a "skin" forms, and turbidity occurs, it indicates that the spawn itself contains contaminating microorganisms; if the spawn block sinks, or only a very thin mycelial layer grows after a long delay, it indicates that the spawn has weak vitality; if the mycelium around the liquid surface grows quickly and is dense white and cotton-like, it indicates that the spawn has strong vitality. 4. Sawdust bottle cultivation assessment and cycle yield assessment
The method for bottle cultivation assessment is similar to that for cultivation production. Pack the sawdust culture material somewhat loosely, appropriately increase the moisture, inoculate the spawn to be assessed into the medium, keep good records, and culture at a constant 26°C for 15 days. Then lower the temperature to 15-20°C and provide good scattered light conditions, and cultivate for about another half month. Then fruiting body primordia and a small number of fruiting bodies will appear on the bottle wall and the surface of the material. If no contaminating microorganisms or abnormal phenomena are found, then inoculate the spawn onto wood logs for cycle yield assessment. If the fruiting bodies grow vigorously, with high yield and good quality, possess the characteristics of a fine variety, and no contaminating microorganisms are mixed in, it indicates that the spawn is reliable and can be preserved and put into large-scale production.
Practice has proven that the above methods are relatively scientific and can screen out fine spawn for users to choose and use.