Secondary bagging of edible fungi can effectively prevent infection

In the production of shiitake mushrooms (Lentinula edodes), lion's mane mushrooms (Hericium erinaceus), wood ear mushrooms (Auricularia heimuer) and others, the secondary bagging technique is applied (that is, when the mushroom logs come out of the sterilization chamber, an extra layer of thin film bag is put on, so that the mushroom logs inside the bag do not come into direct contact with the ambient air), which effectively solves the problem of cross-infection during the log production process and keeps the strain survival rate above 97%. The technique is described below:

1. Preparation of the outer bag The outer bag should preferably be a low-density polyethylene film bag with a circumference 2 cm larger than the inner bag, a length 5 cm longer than the inner bag, and a thickness of 0.2 silk, with one end tied and sealed for later use.

2. Timely bagging When the material logs come out of the sterilization chamber after high-temperature sterilization, put the outer bag over each one as it is taken out, twist the bag opening tightly, and carry them into the culture room, then bring the material log temperature down below 25°C. Note: after the material logs come out of the chamber, they must be quickly put into the outer bags to avoid contact with the external environment for too long, which would cause infection by contaminating microorganisms.

3. Inoculation operation During inoculation, untie the outer bag in the inoculation room or inoculation box, push it toward the closed side, and pull it open 4/5 before inoculating; right after inoculation, put the outer bag back on, twist it tightly, and set it in place.

4. Remove the outer bag at the proper time During the culture process, pay attention to the culture room temperature; when the mycelium of the log has grown to about 80%, remove the outer bag and manage it according to conventional production methods.