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How to reduce contamination in edible mushroom inoculation? Five practical methods to help you solve it

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Autumn and winter are the golden seasons for producing edible mushrooms, but every year, inoculation contamination causes economic losses of around 20% for mushroom growers. Using the following 5 methods can reduce the inoculation contamination rate. 1. Bottled fermented material: Fill fermented material 1 to 2 cm deep above the culture medium at 4 cm from the bottle mouth, then sterilize. Its advantages are that contaminating bacteria are less likely to infect the culture medium, and the spawn age is consistent. 2. Wheat grain germination method: Let wheat grains germinate before bottling and sterilizing. That is, let the wheat grains used for spawn germinate all at once, with sprouts 1 to 2 times the length of the wheat grains, then fully sun-dry and store. One day before making the spawn, soak the wheat grains for 12 hours and directly bottle and sterilize. Its advantages are that the process of boiling the wheat grains is omitted, mycelium grows quickly, and there are fewer contaminating bacteria. The contamination rate is 2% to 3%.

Autumn and winter are the golden seasons for producing edible mushrooms, but every year, inoculation contamination causes economic losses of around 20% for mushroom growers. Using the following 5 methods can reduce the inoculation contamination rate.

1. Bottled fermented material: Fill fermented material 1 to 2 cm deep above the culture medium at 4 cm from the bottle mouth, then sterilize. Its advantages are that contaminating bacteria are less likely to infect the culture medium, and the spawn age is consistent.

2. Wheat grain germination method: Let wheat grains germinate before bottling and sterilizing. That is, let the wheat grains used for spawn germinate all at once, with sprouts 1 to 2 times the length of the wheat grains, then fully sun-dry and store. One day before making the spawn, soak the wheat grains for 12 hours and directly bottle and sterilize. Its advantages are that the process of boiling the wheat grains is omitted, mycelium grows quickly, and there are fewer contaminating bacteria. The contamination rate is 2% to 3%.

3. Use liquid culture medium. Traditional spawn cultivation uses solid culture medium. The disadvantages of solid culture medium are slow mycelial growth, inconsistent spawn age, and a high chance of contamination by foreign bacteria. The improved method is to use liquid culture medium, which overcomes the disadvantages of solid culture medium. Spawn cultivated with liquid culture medium can be directly used as cultivation spawn for cultivation, and the contamination rate can be reduced to 2%.

4. Inoculate from the lower part of the stock spawn bottle. The spawn in the lower part of the stock spawn bottle has a short spawn age, strong vitality, and fewer contaminating bacteria. After inoculation, the mycelium colonizes the substrate quickly and the contamination rate is low. It is recommended to discard the upper 1/5 of the stock spawn at the mouth of a normal stock spawn bottle, and the contamination rate can be reduced to 3%.

5. Reasonably arrange the spawn-making season. In hot and humid environments, contaminating bacteria spread quickly. The improved method is to inoculate when the temperature is relatively low, such as in the morning or at night. If inoculation is done indoors with air conditioning, the effect is even better.

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