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What are the methods for preventing and controlling the degeneration of Agaricus bisporus (button mushroom) spawn

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Preventing spawn degeneration can increase yield, reduce the occurrence and development of pests and diseases, and cut down the amount of pesticides used, which is the foundation for producing organic button mushrooms. 1. The ambient temperature for culturing spawn must not exceed 28°C, and properly cultured spawn should not be stored at room temperature for more than half a month. 2. Properly master the time for mycelium to emerge from the substrate...

Preventing spawn degeneration can increase yield, reduce the occurrence and development of pests and diseases, and cut down the amount of pesticides used, which is the foundation for producing organic button mushrooms.
1. The ambient temperature for culturing spawn must not exceed 28°C, and properly cultured spawn should not be stored at room temperature for more than half a month.
2. Properly master the time and temperature for mycelium to emerge from the substrate, prevent the destruction of nutrients, and keep the pH at a suitable level.
3. Change the substrate frequently; for example, substrate materials such as wheat straw or rice straw, chicken manure, cottonseed hulls and wheat bran should be used alternately.
4. Do not use up the stock culture all at once; take it out in portions and store the remainder at low temperature to prevent too many subcultures.
5. Each year, select large, normally growing, robust fruiting bodies free of pests and diseases and carry out a tissue isolation once, selecting the superior and eliminating the inferior, so as to expand the inoculated culture.

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