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Detailed Explanation of the Key Techniques for Cultivating White Jelly Fungus (Tremella fuciformis) on Sawdust

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How do you cultivate white jelly fungus (Tremella fuciformis) on sawdust? Please explain the cultivation method. To cultivate white jelly fungus on sawdust, you can refer to the following method: 1. Material preparation. It is best to use sawdust from broadleaf poplar, London plane, plum, persimmon, etc. The sawdust should be ground as fine as rice bran. Avoid tree species that contain aromatic oils and other fungicidal substances. 2. Culture medium. The formula for the white jelly fungus culture medium is:...

How do you cultivate white jelly fungus (Tremella fuciformis) on sawdust? Please explain the cultivation method


To cultivate white jelly fungus on sawdust, you can refer to the following method:
    1. Material preparation. It is best to use sawdust from broadleaf poplar, London plane, plum, persimmon, etc. The sawdust should be ground as fine as rice bran. Avoid tree species that contain aromatic oils and other fungicidal substances.  
    2. Culture medium. The formula for the white jelly fungus culture medium: 66.5 kg dry sawdust, 30 kg wheat bran or rice bran, 1 kg sucrose, 1 kg gypsum, 0.5 kg magnesium sulfate, 1 kg soybean. When preparing, first soak the soybean in water for 6 hours to 12 hours, grind it into soybean milk, mix it with the sucrose and magnesium sulfate in water and stir well, then mix the sawdust, wheat bran and gypsum evenly and pour into the mixed liquid. Finally, put the prepared culture material into a wide-mouth bottle, fill it full and press it firm. In the middle of the bottle mouth, make an inoculation hole 2 cm deep and 1.5 cm in diameter. Wrap the bottle mouth with plastic film and kraft paper, and tie it tightly with a rubber band.  
    3. Inoculation. Place the bottles filled with culture material into a steamer and steam for 6 hours for high-temperature sterilization. After cooling, place them in the inoculation box. Also place the inoculation spoon, inoculation knife and alcohol lamp inside, then use potassium permanganate to disinfect the space. When inoculating, use the inoculation spoon to pick up white jelly fungus stock spawn about the size of a peanut, pass it through the flame of the alcohol lamp, place it into the inoculation hole, and press it gently firm. Wrap with plastic film and kraft paper, and tie the bottle mouth tightly with a rubber band. One bottle of 500 g to 750 g of stock spawn can inoculate 50 bottles to 80 cultivation bottles.  
    4. Management. After inoculation, the cultivation bottles should be promptly moved into a cultivation room at 20°C to 28°C for culturing. Set up bed frames indoors, divided into 4 layers to 6 layers, with a spacing of 40 cm. Turn them once every three days. After 3 days, white mycelium appears at the inoculation hole. After 8 days to 10 days, the mycelium spreads downward about 3 cm in the bottle. At this point, the film at the bottle mouth can be removed, and a paper tube made of kraft paper can be placed around the bottle mouth, extending about 3 cm above the bottle mouth. After another 5 days to 10 days, the mycelium changes from tender to thick and strong. At this time, the indoor temperature is best at 23°C to 25°C, and the relative air humidity should be controlled at about 70%. After the fruiting bodies appear, the relative air humidity should be controlled at 75% to 80%. When the white jelly fungus grows to about 4 cm to 5 cm in diameter, the paper tube can be opened, and the cultivation bottles can be arranged on bed frames covered with plastic film. The relative air humidity should be maintained at about 95% to allow the mycelium to differentiate, and ventilation should be strengthened. When the white jelly fungus grows to 10 cm to 12 cm, it can be harvested.  
    5. Harvesting. Use a sharp knife to cut off the whole white jelly fungus at the base, and use small scissors to cut off the light yellow base. Then wash away impurities with clean water, spread out to sun-dry, or dry over a low fire at 40°C to 60°C.  
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