In vitro propagation of clivia makes factory-scale production possible
In vitro propagation of clivia
(1) Plant name: Clivia miniata
In vitro propagation of clivia
(1) Plant name: Clivia miniata
(2) Material type: seed embryo
(3) Culture conditions: MS as the basic medium. (1) Callus induction medium: MS + 2,4—D 1 mg/L (same units below) + hydrolyzed protein 500; (2) Shoot differentiation medium: MS + BA 2 + NAA 0.005 + hydrolyzed protein 500; (3) Rooting medium: 1/2 MS + NAA 0.1 + KT 0.5 + LBA 0.5 + activated carbon 5 g/L. All the above media were supplemented with 3% sucrose and 0.8% agar, pH 5.8. Culture temperature (24±2)°C; light requirements are not strict.
(4) Growth and differentiation
1. Callus induction. Strictly sterilized, excised clivia seed embryos were inoculated onto callus induction medium (1). After 20 days the radicle swelled, the leaf primordia began to sprout, and the small leaves that formed soon stopped growing; then the hypocotyl region began to swell, gradually forming pale yellow callus, after which part of the callus turned green.
2. Shoot differentiation and proliferation. The greened callus was transferred to differentiation medium (2) for continued culture. The parts that had turned green gradually differentiated into small shoots, with each piece of callus forming anywhere from 3 to 20 small shoots. The shoot-differentiating callus could also be continually cut and subcultured, thus yielding large numbers of test-tube plantlets.
3. Transplanting. Green plantlets with 2—3 leaves were cut off and inoculated onto rooting medium (3). After 1 month of culture, yellow conical root tips emerged at the lower cut end, 1-5 per plant, and the root system was thick and strong. It is best to open the bottle stopper and harden the plantlets for 3 days. They were then transplanted into vermiculite, avoiding direct strong sunlight, with the temperature controlled at 20°C—25°C and air humidity maintained at around 85%; the survival rate was over 90%.
(5) Significance and progress. Clivia is a perennial herbaceous plant of the genus Clivia in the Amaryllidaceae. Its flowering period lasts as long as 3 months and coincides with New Year's Day and the Spring Festival. Its broad, thick leaves, evergreen or dark green throughout the year and spreading evenly and symmetrically on both sides, have extremely high ornamental value and also high economic value. At present, clivia is mostly propagated by division and by seed, with a low multiplication rate; when popularizing newly bred cultivars, this is even more slow, greatly limiting the production and development of clivia. In recent years we have studied rapid propagation using clivia seed embryos in order to raise its rapid propagation coefficient. Through in vitro propagation of clivia, the long-standing problems of its slow growth and few tillers have been preliminarily solved, providing a feasible route for factory-scale production of clivia. A feasible route.
(3) Culture conditions: MS as the basic medium. (1) Callus induction medium: MS + 2,4—D 1 mg/L (same units below) + hydrolyzed protein 500; (2) Shoot differentiation medium: MS + BA 2 + NAA 0.005 + hydrolyzed protein 500; (3) Rooting medium: 1/2 MS + NAA 0.1 + KT 0.5 + LBA 0.5 + activated carbon 5 g/L. All the above media were supplemented with 3% sucrose and 0.8% agar, pH 5.8. Culture temperature (24±2)°C; light requirements are not strict.
(4) Growth and differentiation
1. Callus induction. Strictly sterilized, excised clivia seed embryos were inoculated onto callus induction medium (1). After 20 days the radicle swelled, the leaf primordia began to sprout, and the small leaves that formed soon stopped growing; then the hypocotyl region began to swell, gradually forming pale yellow callus, after which part of the callus turned green.
2. Shoot differentiation and proliferation. The greened callus was transferred to differentiation medium (2) for continued culture. The parts that had turned green gradually differentiated into small shoots, with each piece of callus forming anywhere from 3 to 20 small shoots. The shoot-differentiating callus could also be continually cut and subcultured, thus yielding large numbers of test-tube plantlets.
3. Transplanting. Green plantlets with 2—3 leaves were cut off and inoculated onto rooting medium (3). After 1 month of culture, yellow conical root tips emerged at the lower cut end, 1-5 per plant, and the root system was thick and strong. It is best to open the bottle stopper and harden the plantlets for 3 days. They were then transplanted into vermiculite, avoiding direct strong sunlight, with the temperature controlled at 20°C—25°C and air humidity maintained at around 85%; the survival rate was over 90%.
(5) Significance and progress. Clivia is a perennial herbaceous plant of the genus Clivia in the Amaryllidaceae. Its flowering period lasts as long as 3 months and coincides with New Year's Day and the Spring Festival. Its broad, thick leaves, evergreen or dark green throughout the year and spreading evenly and symmetrically on both sides, have extremely high ornamental value and also high economic value. At present, clivia is mostly propagated by division and by seed, with a low multiplication rate; when popularizing newly bred cultivars, this is even more slow, greatly limiting the production and development of clivia. In recent years we have studied rapid propagation using clivia seed embryos in order to raise its rapid propagation coefficient. Through in vitro propagation of clivia, the long-standing problems of its slow growth and few tillers have been preliminarily solved, providing a feasible route for factory-scale production of clivia. A feasible route.