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What are the causes of contamination in Lingzhi (Ganoderma) spawning?
1. Damage contamination (1) Container damage: mechanical damage caused by failure to handle with care during filling, sterilization, inoculation, transport and cultivation. (2) Air-pocket damage: rapid heating, pressure rise or rapid cooling during sterilization; excessive exhaust speed, causing bags to swell with air and rupture. 2. Cotton plug contamination (1) Cotton plug contamination: the test tube mouth is stained with culture medium or the bottle (bag) mouth is not clean, or the culture medium comes into contact with the cotton plug. (2) Cotton plug dampened: improper stacking during sterilization, too much water added, or unreasonably built sterilization stove, or culture
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Introduction to the methods of germination and sowing of Gastrodia mushroom spawn
1. Types of germination fungi The germination fungi of Gastrodia seeds reported so far all belong to 4 species of fungi in the Tricholomataceae family, Mycena genus. Their names are: first, Mycena osmarioides; second, Mycena orchidicola; third, Mycena dendrobii; fourth, Mycena anoectochila. In 1999, we isolated and cultured another type of fungus for Gastrodia seed germination, which is Trametes lactinea of the Polyporaceae family. This fungus is superior to the above four fungi in production and application during the higher temperature season. 2. Production technology of germination fungi Isolation and culture of the mother spawn of germination fungi: in Gastrodia cultivation sites
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