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Induced biochemical responses in tea plant leaves after infection by the tea blister blight pathogen
The activities of enzymes such as phenylalanine ammonia-lyase, polyphenol oxidase and peroxidase, as well as the levels of proteins, proline and phenolic compounds in the leaves, were studied after tea plant leaves were infected by the tea blister blight pathogen. The study showed that in diseased leaves the activity of phenylalanine ammonia-lyase decreased, while the activities of peroxidase and polyphenol oxidase increased. Analysis of peroxidase isoenzymes by polyacrylamide gel electrophoresis using benzidine as substrate showed that healthy tea plant leaves had 4 isoenzymes, whereas