How to dilute semen in artificial insemination of pigs
Artificial insemination methods for pigs - dilution of semen
Step one: prepare a dedicated diluent, such as KIEV, BTS, 20rpva, etc. Step two: determine the number of dilution portions based on sperm density, usually diluting 6 to 8 times. Step three: carefully pour the semen into the diluent, which should be at the same temperature as the semen (± <1 degree). Step four: the diluted semen can then be used for insemination of sows, or it can be stored; a disposable plastic bag (bottle) can hold 60 to 80 milliliters of semen, which is exactly the amount for 1 insemination, and a label should be attached, then it is stored in a constant-temperature box at 17 degrees. For fresh semen, if it is used immediately after collection, a 5% glucose solution or isotonic glucose saline (37 degrees) can be used for 6 to 8 times dilution.