Sharing the results of a swine fever vaccine trial, several sets of data for reference
Sharing a few test results for swine fever vaccines
This post was last edited by xueyoumi on 2009-11-22 10:20
To avoid unnecessary arguments about spleen-lymph node vaccines, tissue vaccines, etc., to avoid getting flamed by everyone, and to avoid any suspicion of advertising, I won't disclose what vaccine each of these three lines represents. I hope you can help me take a look and see which line represents the better vaccine. The red one, the yellow one, or the purple one? Just look at the chart.
xueyoumi added the following content on 2009-11-22 10:18
"1-7" means day 7 after the first vaccination, and so on.
xueyoumi added the following content on 2009-11-22 10:20
We give the first vaccination at 25 days of age and the second vaccination at 60 days of age. Attachment: You need to log in to download or view attachments. No account? Register
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For breeding pigs, the blocking rate should ideally be above 50; for commercial pigs, above 40, and reaching 50 would be good. I don't really understand the coefficient of variation!
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The purple one is good,
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Could you explain the blocking value and coefficient of variation?
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Reply to post 5# xxc1963
With the IDEXX swine fever antibody detection kit, a blocking value below 30 is negative, indicating no antibodies; between 30 and 40 is suspicious; above 40 is positive, indicating the presence of antibodies. The higher the value, the higher the antibody level can be understood to be. As for exactly how high is appropriate, there are many opinions.
xueyoumi added the following content on 2009-11-23 21:32
The coefficient of variation reflects the uniformity of the herd's antibody levels. A small coefficient of variation indicates that the herd's antibody levels are uniform. Of course, the more uniform the antibody levels, the better.
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Let me ask the original poster a prerequisite question: how did you determine the timing of the first vaccination at 25 days of age? What were the maternal antibody levels before the first vaccination, and were the experimental groups completely consistent? Judging from the average antibody blocking rate 7 days after vaccination that you provided, the timing of the first vaccination may have been a bit early, which would affect the subsequent immune evaluation. Based on your results alone, yellow is better than the other two, but there is a problem: the antibody change curve after the second vaccination is not easy to explain, with no obvious rise, and antibodies decline after 45 days. Is this due to sampling effects, or is there something else that remains to be resolved?
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This post was last edited by xueyoumi on 2009-11-30 17:52
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Due to the presence of maternal antibodies, after the first swine fever vaccination is given, some of the antigen will definitely be neutralized by maternal antibodies, so the decline in antibody levels can be explained. According to what some teachers say, antibody levels should reach their lowest around day 15 after vaccination, and then rise afterward. The flaw in our trial is that we collected blood for testing 15 days after the first vaccination, so we don't know what the level was at that time.
We are now doing a supplementary trial, adding blood collection at 15 days after the first vaccination. Preliminary data show that antibody levels are indeed lower at 15 days after the first vaccination. In about half a month, once all the results are out, I will post the data again.
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Could the original poster disclose what type of vaccine each of these lines represents? No need to mention the manufacturer.
[ts]wdeming added the following content on 2009-11-30 18:05[/ts]
My farm has always used spleen-lymph node vaccine, and I feel the effect is still okay
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This post was last edited by xueyoumi on 2009-11-30 19:45
To avoid unnecessary arguments about spleen-lymph node vaccines, tissue vaccines, etc., to avoid getting flamed by everyone, and to avoid any suspicion of advertising, I won't disclose what vaccine each of these three lines represents. I hope you can help me take a look and see which line represents the better vaccine. The red one, the yellow one, or the purple one? Just look at the chart.
xueyoumi added the following content on 2009-11-22 10:18
"1-7" means day 7 after the first vaccination, and so on.
xueyoumi added the following content on 2009-11-22 10:20
We give the first vaccination at 25 days of age and the second vaccination at 60 days of age. Attachment: You need to log in to download or view attachments. No account? Register
--------------------------------------
For breeding pigs, the blocking rate should ideally be above 50; for commercial pigs, above 40, and reaching 50 would be good. I don't really understand the coefficient of variation!
--------------------------------------
For breeding pigs, the blocking rate should ideally be above 50; for commercial pigs, above 40, and reaching 50 would be good. I don't really understand the coefficient of variation!Aren't your requirements too low? The coefficient of variation reflects the uniformity of the herd's antibody levels. A small coefficient of variation indicates that the herd's antibody levels are uniform.
taiba posted on 2009-11-22 21:00
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The purple one is good,
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Could you explain the blocking value and coefficient of variation?
--------------------------------------
Reply to post 5# xxc1963
With the IDEXX swine fever antibody detection kit, a blocking value below 30 is negative, indicating no antibodies; between 30 and 40 is suspicious; above 40 is positive, indicating the presence of antibodies. The higher the value, the higher the antibody level can be understood to be. As for exactly how high is appropriate, there are many opinions.
xueyoumi added the following content on 2009-11-23 21:32
The coefficient of variation reflects the uniformity of the herd's antibody levels. A small coefficient of variation indicates that the herd's antibody levels are uniform. Of course, the more uniform the antibody levels, the better.
--------------------------------------
Let me ask the original poster a prerequisite question: how did you determine the timing of the first vaccination at 25 days of age? What were the maternal antibody levels before the first vaccination, and were the experimental groups completely consistent? Judging from the average antibody blocking rate 7 days after vaccination that you provided, the timing of the first vaccination may have been a bit early, which would affect the subsequent immune evaluation. Based on your results alone, yellow is better than the other two, but there is a problem: the antibody change curve after the second vaccination is not easy to explain, with no obvious rise, and antibodies decline after 45 days. Is this due to sampling effects, or is there something else that remains to be resolved?
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This post was last edited by xueyoumi on 2009-11-30 17:52
Let me ask the original poster a prerequisite question: how did you determine the timing of the first vaccination at 25 days of age? What were the maternal antibody levels before the first vaccination, and were the experimental groups completely consistent? Judging from what you...We wean at 16-18 days. Regarding the decline in antibodies after the first vaccination, we were also puzzled at one point. According to our monitoring, by 29 days of age, the average swine fever antibody blocking value had already dropped to 42, so vaccinating at 25 days of age is not early. Let me show you our monitoring data. Attachment: You need to log in to download or view attachments. No account? Register
xiaorenwu posted on 2009-11-30 14:01
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Due to the presence of maternal antibodies, after the first swine fever vaccination is given, some of the antigen will definitely be neutralized by maternal antibodies, so the decline in antibody levels can be explained. According to what some teachers say, antibody levels should reach their lowest around day 15 after vaccination, and then rise afterward. The flaw in our trial is that we collected blood for testing 15 days after the first vaccination, so we don't know what the level was at that time.
We are now doing a supplementary trial, adding blood collection at 15 days after the first vaccination. Preliminary data show that antibody levels are indeed lower at 15 days after the first vaccination. In about half a month, once all the results are out, I will post the data again.
--------------------------------------
Could the original poster disclose what type of vaccine each of these lines represents? No need to mention the manufacturer.
[ts]wdeming added the following content on 2009-11-30 18:05[/ts]
My farm has always used spleen-lymph node vaccine, and I feel the effect is still okay
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This post was last edited by xueyoumi on 2009-11-30 19:45
Could the original poster disclose what type of vaccine each of these lines represents? No need to mention the manufacturer.Then let me disclose it: the yellow line represents Company A's cell vaccine, the red line represents Company B's spleen-lymph node vaccine, and the purple line represents Company B's cell vaccine. The red and purple ones are from the same company.
[ts]wdeming added the following content on 2009-11-30 18:05[/ts]
wdeming posted on 2009-11-30 18:03