What books are there on nutrition science? Teacher Lu Dexun introduced several
Teacher Lu Dexun introduces several books on nutrition science to everyone
1. Molecular Nutrition - National Higher Education Supporting Textbook Author: Zhang Changhao, Editor-in-Chief
Publisher: People's Medical Publishing House
Publication time: 2006-7-1 Word count: 469000 Edition: 1 Page count: 251 Printing time: 2006/07/01 ISBN: 9787117077705 Content introduction
The book is divided into 5 chapters. Chapter 1 briefly introduces the concept of gene expression and the basic theory of gene expression regulation. Because the regulation of gene expression by nutrients involves these basic theories of molecular biology, introducing them at the beginning of the book helps lay a foundation for learning the knowledge that follows. Chapter 2 covers the regulation of gene expression by nutrients and their effects on genome structure and stability, which is one of the main research areas of molecular nutrition. In Chapter 3, using various nutrients as examples, the regulation of gene expression by each nutrient is introduced in detail, because although nutrient regulation of gene expression has some common features, each also has its own characteristics. Chapter 4 covers the effects of gene polymorphism on nutrient absorption, metabolism and utilization, which is another main research area of molecular nutrition. Chapter 5, on the basis of the above chapters, explores the patterns and mechanisms of the effects of nutrient-gene interactions on health, namely the role of nutrient-gene interactions in the occurrence of disease. This book contains a great deal of content and involves much knowledge of molecular biology. After the main text, major reference materials are attached, which can be used by students for further reading and by teachers for reference in teaching. An English-Chinese index is also attached at the end of the book for lookup. This book can be used as a textbook for undergraduate and graduate students in nutrition and food majors. Table of contents Introduction
Chapter 1 The concept of gene expression and the basic theory of gene expression regulation
Section 1 Basic concepts of gene expression
Section 2 Basic theory of gene expression regulation
Section 3 The significance of gene expression regulation
Chapter 2 Regulation of gene expression by nutrients and effects on genome structure and stability
Section 1 Regulation of gene expression by nutrients
Section 2 Effects of nutrients on genome structure and stability
Chapter 3 Regulation of gene expression by various nutrients
Section 1 Regulation of gene expression by protein
Section 2 Regulation of gene expression by fat
Section 3 Regulation of gene expression by carbohydrates
Section 4 Regulation of gene expression by calcium
Section 5 Regulation of gene expression by iron
Section 6 Regulation of gene expression by zinc
Section 7 Regulation of gene expression by selenium
Section 8 Regulation of gene expression by chromium
Section 9 Regulation of gene expression by vitamin A
Section 10 Regulation of gene expression by vitamin D
Section 11 Regulation of gene expression by vitamin E
Section 12 Regulation of gene expression by biotin
Chapter 4 Effects of gene polymorphism on nutrient absorption, metabolism and utilization
Section 1 Effects of gene polymorphism on folate metabolism
Section 2 Effects of vitamin D receptor gene polymorphism on calcium metabolism
Section 3 Effects of gene polymorphism on cholesterol metabolism
Section 4 Effects of gene polymorphism on iron metabolism
Section 5 Gene polymorphism and lactose intolerance
Chapter 5 The role of nutrient-gene interactions in the occurrence of disease
Section 1 Overview
Section 2 Effects of early-life nutrition on obesity-related diseases in adulthood
Section 3 Effects of nutrition-gene interactions on hyperlipidemia
Section 4 Effects of nutrient-gene interactions on the occurrence of obesity
Section 5 Effects of nutrient-gene interactions on salt-sensitive hypertension
Section 6 Effects of nutrient-gene interactions on the occurrence of diabetes
References
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2. Molecular Nutrition Author: (US) J. Zempleni, (Germany) H. Daniel, edited, translated by Luo Xugang et al.
Publisher: Science Press
Publication time: 2008-5-1 Word count: 614000 Edition: 1 Page count: 414 Printing time: 2008/05/01 Format: Large 16 mo Printing count: 1 Paper: Offset paper ISBN: 9787030173713 Content introduction
Molecular nutrition is the fastest-growing science in the field of nutrition. This book is currently the only Chinese translation published in China on research progress in the field of molecular nutrition. The book reviews the latest and most important research results on molecular nutrition published by first-class international experts. The book is divided into 24 chapters, describing in detail the research methods of molecular nutrition, the dynamic homeostasis of nutrients in cells, cell proliferation and apoptosis, signal transduction, gene expression and proteolysis, the physiological roles of nucleic acids and events at the molecular level, and the molecular mechanisms of genetically modified foods and food allergies.
This book is faithful to the original text and reflects the style and flavor of the original book to the greatest extent. It can be used as a reference book for teaching and research personnel and graduate students in nutrition, animal nutrition, biochemistry, molecular biology and pharmaceutical sciences. Table of contents
Translator's preface
Foreword
Part 1 Methods of molecular nutrition research
1 Genome and post-genome
Ji Zhang
2 Prospects for post-genomic nutrition research
Hannelore Daniel
Part 2 Cellular nutrient homeostasis, cell proliferation and apoptosis
3 Molecular physiology of plasma membrane transport proteins for organic nutrients
Hannelore Daniel
4 Intracellular transport and compartmentalization of vitamins and their physiologically active forms
Donald B.McCormick
5 Nutrient homeostasis in proliferating cells
Janos Zempleni
6 Nutrition and apoptosis
John C.Mathers
Part 3 Roles of nutrients in signal transduction, gene expression and proteolysis
7 Regulation of gene expression by glucose in mammals
Fabienne Foufelle and Pascal Ferre
8 Amino acid-dependent transcriptional regulation in NgL animal cells
Michael S. Kilberg, Van Leung-Pineda and Chin Chen
9 Fatty acids and gene expression
Ulrike Beisiegel, Joerg Heeren and Frank Schnieders
10 The role of retinoic acid receptors and retinoid X receptors in the molecular mechanism regulating the action of vitamin A
Dianne R. Soprano and Kenneth J. Soprano
11 Regulation of gene expression by biotin, vitamin B6 and vitamin C
Krishnamurti Dakshinamurti
12 Selenium and vitamin E
Alexandra Fischer, Josef Pallauf, Jonathan Maj ewicz, Anne Marie Minihane and Gerald Rimbach
13 Sphingolipids: new strategies for cancer treatment and prevention
Eva M.Schmelz
14 Health-promoting effects of isoflavones in food
Thomas M. Badger, Martin J. J. Ronis and Nianbai Fang
15 Ubiquitination and proteasome-dependent proteolytic mechanisms in skeletal muscle
Didier AttaiX, Lydie Combarct, Anthony J. Kee and Daniel Taulandier
Part 4 Nucleic acids and nucleic acid-binding compounds
16 Diet, DNA methylation and cancer
Judith K.Christman
17 Biotinylation of histones in human cells
Janos Zempleni
18 Niacin nutritional status, poly(ADP-ribose) metabolism and genome instability
Jennifer C. Spronck and James B. Kirkland
Part 5 Effects of molecular activity on physiology
19 Assembly of plasma lipoproteins that transport triglycerides
Joan A.Higgins
20 Regulation of cellular cholesterol
Ji-Young Lee, Susan H. Mitmesser and Timothy P. Carr
21 Year 2002: Assessment of the effect of nutrition on the incidence of cataracts
Allen Tavlor and Mark Siegal
22 Nutrition and immune function
Parveen Yaqoob and Philip C. Calder
Part 6 Food
23 Molecular mechanisms of food allergy
J. Steven Stanley and Gary A. Bannon
24 Safety evaluation of genetically modified foods
Steve L.Taylor
Index Book excerpt Illustrations
1 Genome and post-genome Ji Zhang
(1 Department of Pathology and Microbiology, University of Nebraska Medical Center; 2 Human Molecular Genetics Center, Munroe-Meyer Institute, University of Nebraska Medical Center, Omaha, Nebraska, USA)
Introduction
In recent years, tremendous progress in the field of genome research has driven the precise determination of the molecular mechanisms of human health and disease, providing great potential for improving health, reducing morbidity and mortality, and preventing disease. Understanding the molecular mechanisms by which healthy adults respond differently to nutrient intake will greatly advance the development of nutrition science, so it is quite important for nutritionists to acquire knowledge of the necessary techniques and resources for genome research. Originally, genomics referred to the scientific principles of mapping, sequencing and analyzing an organism's genome, that is, the entire set of genes and chromosomes. At present, the research focus of genomics has shifted from structural analysis of the genome (structural genomics) to functional analysis of the genome (functional genomics). The goal of structural genomics is to construct high-resolution genetic, physical and transcriptional maps of an organism and ultimately determine its entire DNA sequence. However, functional genomics represents a new stage of genome research and refers to developing innovative technologies on the basis of large amounts of structural genomics information. The first part of this chapter will focus on the tools and reagents used in structural genomics; the second part will focus on DNA microarray technology, which is the representative of today's functional genomics.
Structural genomics
Genome, genetic mapping and physical mapping
The human genome contains nearly 3 billion nucleotide base pairs and carries the genetic code for 30000 to 100000 genes. The diploid genome DNA constitutes 22 pairs of autosomes and two sex chromosomes. Each chromosome contains a linear DNA molecule, which is characterized by containing 3 functional elements that are necessary for successful chromosome replication during cell division: 1 self-replicating sequences; 2 centromeres, namely the attachment points of the spindle during mitosis or meiosis; 3 telomeres, which ensure the complete replication of the terminal chromatids. A genome map is defined as the relative positions of different loci (genes, regulatory sequences, polymorphism marker sequences, etc.) in a DNA molecule. There are two different methods for mapping genome loci, namely genetic mapping and physical mapping.
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3.Integrated Applied PhysiologyAuthor: Feng Zhiqiang, Editor-in-Chief
Publisher: People's Military Medical Press
Publication time: 2006-5-1 Word count: 540000 Edition: 1 Page count: 345 Printing time: 2006/05/01 Format: Printing count: Paper: Offset paper ISBN: 9787509102237 Content introduction
Under the guidance of materialist dialectics, this book integrates physiological knowledge with related research progress and relevant humanities knowledge to form a unique knowledge framework. The book consists of 6 chapters and 55 sections in total: the development of physiology, the basic characteristics of life, homeostasis mechanisms, cell function, the functional activities and coordination mechanisms of the body's various systems, and the body's protective mechanisms. Each section explains a relatively complex physiological issue in an integrated form, with both a certain breadth and corresponding depth; at the same time, it also focuses on combining the basic theories of physiology with certain clinical changes or research progress; on the premise of taking physiological knowledge as the core, it proposes new hypotheses or issues not yet clarified, and especially the introduction of the thoughts and spirit of some Nobel Prize winners in Physiology or Medicine has the effect of enlightening readers' thinking. This book reorganizes physiological knowledge with a novel conception, and its content is comprehensive and substantial, with highly integrated figures mainly designed by the author. This book is mainly used for graduate students and undergraduate students of various majors in medical colleges to learn physiological knowledge and conduct thinking training on complex issues, and it also has reference value for teachers engaged in basic and clinical teaching. Table of contents
Chapter 1 The development of physiology
Section 1 The development of physiology and the role of materialist dialectics in it
1. Ancient understanding of human physiology
2. The development of modern physiology
3. The development of contemporary physiology
4. The role of materialist dialectics in the development of physiology
5. Ideas that should be upheld and issues that should be noted in physiology research
Section 2 The development of physiology in China
1. Overview of the development of physiology in China
2. China's contributions to the development of physiology
3. Contributions of the older generation of physiologists to the development of physiology in China
4. The development of China's physiology cause is closely linked to the fate of the motherland
Section 3 The macro level and micro level of physiology research
1. Content of macro-level and micro-level research
II. Progress and achievements in research at the macro and micro levels
III. The complementary role of research at the macro and micro levels
Section 4 Methods and techniques in physiology research
I. Methods of physiology research
II. Intracellular recording techniques and extracellular recording techniques
III. Development of the conditions for physiology research
Chapter 2 Basic characteristics of life
Section 1 Metabolism
I. Metabolism and its two aspects
II. The relationship between metabolism and the functions of the body's various systems
Section 2 Excitability and its manifestations
I. The concept of excitability
II. The manifestations of excitability
Section 3 The origin and movement of life
I. The origin of life
II. Individual growth and development and its regulation
III. The meaning of life and attitudes toward life
Section 4 Information transmission in life activities
I. Major events in the study of cellular information transmission
II. Informational substances in the body and their movement
III. The role and mechanisms of transmembrane information transmission
IV. Information transmission in life activities and its characteristics
Section 5 Coordination between the body and its environment and its mechanisms
Chapter 3 Homeostatic mechanisms
Section 1 Regulation of the body's functional activities and automatic control
Section 2 The physicochemical properties of intracellular fluid and extracellular fluid and the mechanisms that stabilize them
Section 3 Acid-base balance in body fluids and the mechanisms that maintain it
Section 4 The stabilization mechanism of plasma osmotic pressure and changes in body fluid osmotic pressure
Section 5 The stabilization mechanism of the body's blood pressure
Section 6 Absorption and excretion functions and their role in homeostasis
Section 7 The stabilization mechanism of the body's body temperature
Chapter 4 Cell functions
Section 1 Active transport and passive transport functions of the cell membrane
Section 2 Ion channels and membrane currents in the cell membrane
Section 3 Activity changes in nerve cells and their mechanisms
Section 4 The process and mechanisms of synaptic transmission
Section 5 The motor function of muscle cells
Section 6 The function, production and destruction of red blood cells
Section 7 Electrical activity and physiological properties of cardiac autorhythmic cells and non-autorhythmic cells
Section 8 The role of ions in the activity of ventricular myocardial cells
Chapter 5 The functional activities of the body's various systems and their coordination mechanisms
Section 1 The heart's blood-pumping function and its changes
Section 2 The formation mechanism of arterial blood pressure
Section 3 The functional activity and regulation of microcirculation
Section 4 Regulation of cardiovascular activity
Section 5 The formation and regulation of respiratory movement
Section 6 Gas exchange and transport
Section 7 Digestive function and its coordination
Section 8 The mechanisms underlying hunger and satiety
Section 9 The movement and regulation of energy substances in the body
Section 10 The process of urine formation and excretion and its regulation
Section 11 Regulation of the body's autonomic functional activities
Section 12 The process and mechanisms of vision
Section 13 The process and mechanisms of hearing
Section 14 Pain and analgesia
Section 15 Other sensory functions of the body
Section 16 Changes in the body's functional activities during exercise and their regulation
Section 17 Stretch reflex and reverse stretch reflex
Section 18 The mechanisms maintaining the body's posture and movement
Section 19 Wakefulness and sleep
Section 20 Memory and forgetting
Section 21 Unconditioned reflexes and conditioned reflexes
Section 22 Regulation of the body's functional activities by the cerebral cortex
Section 23 The effects and regulation of endocrine and exocrine secretion
Section 24 The effects and mechanisms of hormones on receptor regulation
Section 25 Synergistic and antagonistic effects of hormones
Section 26 The effects of sex hormones and the regulation of their secretion
Chapter 6 The body's protective mechanisms
Section 1 The body's immune function
Section 2 Blood coagulation and anticoagulation mechanisms
Section 3 Damaging factors in the stomach and the stomach's self-protective effects
Section 4 The body's response to emergencies and its mechanisms
Section 5 Damaging factors in the environment and the body's protective mechanisms
References
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These books are all good, especially the third one, "Integrative Physiology," which is extremely helpful for a comprehensive understanding of the animal body!
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4.Molecular Biology and GenomicsAuthor: Edited by (Germany) Muelhardt
Publisher: Science Press
Publication time: 2007-7-1 Word count: 421000 Edition: 1 Page count: 257 Printing time: 2007/07/01 Format: Printing: Paper: Offset paper ISBN: 9787030193896 Binding: Hardcover Category: Books >> Natural Science >> Biological Science >> Genetics
Content introduction
At present, a major confusion faced by laboratory personnel is how to use the correct tools and methods to explain the structure, function and expression interpretation of the relevant proteins encoded by the genome. "Molecular Biology and Genomics" by Muelhardt of the University of Marburg in Germany provides everyone with many useful laboratory tips and techniques that can greatly improve the success rate and accuracy of experiments. This laboratory guide briefly and clearly introduces the various modern methods and various routine methods involved in molecular biology and genomics, and discusses the advantages and disadvantages of each method.
As one of the "Experimenter" series, this book adheres to the consistent style of the series and remains a very practical laboratory manual. In a concise and clear style, using more than 100 charts, it provides a large number of practical tips for the broad community of molecular biology and genomics laboratory personnel to supplement and improve basic knowledge, thereby greatly improving the accuracy and success rate of experiments.
This book is suitable for laboratory personnel, scientific researchers, teachers, senior undergraduates and graduate students in the fields of molecular biology and genomics. Contents
Introduction
Preface to the first edition
Acknowledgments
1 What is molecular biology?
1.1 Basic knowledge of molecular biology: introducing beginners to the molecular world
1.2 Basic requirements of molecular biology
1.3 Laboratory safety
2 Introduction to basic methods
2.1 Differences in nucleic acids
2.2 Concentration of nucleic acids by precipitation
2.2.1 Ethanol precipitation method
2.2.2 Concentration devices
2.2.3 Freeze drying
2.2.4 Salting out
2.3 Nucleic acid purification
2.3.1 Phenol-chloroform extraction purification method
2.3.2 Polyethylene glycol precipitation method
2.3.3 Protein-binding membrane purification method
2.3.4 Anion exchange column purification method
2.3.5 Glass milk purification method
2.3.6 Cesium chloride density gradient centrifugation method
2.3.7 Dialysis method
2.4 Determination of nucleic acid concentration
2.4.1 Spectrophotometer method
2.4.2 Agarose gel electrophoresis method
2.4.3 Dot quantification method
2.4.4 Fluorescence quantification method
2.4.5 Nucleic acid detector method
2.4.6 Enzyme labeling method
2.5 DNA preparation methods
2.5.1 Small-scale preparation of plasmid DNA
2.5.2 Large-scale preparation of plasmid DNA
2.5.3 Bacterial culture medium
2.5.4 Preparation of phage DNA
2.5.5 Preparation of single-stranded DNA using helper phage
2.5.6 Genomic DNA Preparation
3 Molecular Biology Tools
3.1 Restriction Enzymes
3.1.1 Nomenclature
3.1.2 Activity Assay
3.1.3 Restriction Enzyme Digestion Reaction
3.1.4 Difficulties in Restriction Enzyme Digestion
3.1.5 Guidelines for Restriction Enzyme Digestion
3.2 Gels
3.2.1 Agarose Gels
3.2.2 Recovery of DNA Fragments from Agarose Gels
3.2.3 Polyacrylamide Gels
3.2.4 Recovery of DNA Fragments from Polyacrylamide Gels
3.2.5 Pulsed-Field Gel Electrophoresis
3.2.6 Capillary Electrophoresis
3.3 Blotting
3.3.1 Southern Blotting
3.3.2 Northern Blotting
3.3.3 Dot Blotting and Slot Blotting
4 Polymerase Chain Reaction
4.1 Standard Polymerase Chain Reaction
4.2 Modified Polymerase Chain Reaction
4.2.1 Nested Polymerase Chain Reaction
4.2.2 Multiplex Polymerase Chain Reaction
4.2.3 Long-Range Polymerase Chain Reaction
4.3 Applications of Polymerase Chain Reaction
4.3.1 Reverse Transcription Polymerase Chain Reaction
4.3.2 Rapid Amplification of cDNA Ends
4.3.3 Amplification of Identical Products
4.3.4 Classic Quantitative Polymerase Chain Reaction
4.3.5 Real-Time Quantitative Polymerase Chain Reaction
4.3.6 Inverse Polymerase Chain Reaction
4.3.7 Biotin-RAGE Polymerase Chain Reaction
4.3.8 Modified Primer Mutagenesis
4.3.9 Amplification Refractory Mutation System
4.3.10 In Situ Polymerase Chain Reaction
4.3.11 Cycle Sequencing
4.3.12 cDNA Synthesis
4.3.13 Single-Cell Polymerase Chain Reaction
5 RNA
5.1 RNase Inactivation
5.2 RNA Extraction Methods
5.2.1 One-Step Method
5.2.2 Ethylphenyl Polyethylene Glycol Lysis Method
5.2.3 Basic Information
5.2.4 RNA Concentration Determination
5.3 mRNA Isolation Methods
5.3.1 Purchasing RNA
5.4 Reverse Transcription: cDNA Synthesis
5.5 In Vitro Transcription: RNA Synthesis
5.6 RNA Interference
6 Cloning DNA Fragments
6.1 Basic Elements of Cloning
6.1.1 Vectors
6.1.2 DNA Fragments
6.1.3 Fill-In Reaction
6.1.4 DNA Quantification
6.1.5 Ligation
6.1.6 Cloning PCR Products
6.1.7 Cloning with Recombinase Systems
6.2 Choosing Cloning Vectors
6.2.1 Plasmids
6.2.2 Bacteriophages
6.2.3 Cosmids
6.2.4 P1 Artificial Chromosomes and Bacterial Artificial Chromosomes
6.2.5 Yeast Artificial Chromosomes
6.3 Choosing Bacteria
6.4 Preparation of Competent Cells and Transformation
6.4.1 Calcium Chloride Method
6.4.2 Rubidium Chloride Method
6.4.3 TSS Method
6.4.4 Electroporation
6.4.5 Titer Determination
6.5 Issues Related to Cloning
6.6 Storage of Clones
7 Hybridization: How to Detect DNA
7.1 Preparing Probes
7.1.1 Methods for Preparing Labeled Probes
7.2 Hybridization
7.2.1 Hybridization Buffers
7.2.2 Hybridization Reaction System
7.2.3 Hybridization Temperature
7.2.4 Washing
7.3 Verification of Labeled DNA
7.3.1 Autoradiography
7.3.2 Non-Radioactive Detection Methods
7.4 Screening of Recombinant DNA Libraries
7.4.1 Preparation of DNA Library Plates
7.4.2 Membrane Transfer
7.4.3 Membrane Hybridization
7.4.4 Membrane Exposure
7.4.5 Clone Probing
7.4.6 Future Library Screening
7.5 Two-Hybrid System
8 DNA Analysis
8.1 Sequencing
8.1.1 Radioactive Sequencing
8.1.2 Non-Radioactive Sequencing and Automated Sequencing Units
8.1.3 Pocket-Sized Sequencing
8.1.4 Pyrosequencing
8.1.5 Sequencing Characteristics: Octamers
8.2 Methods for Analyzing DNA Mutations
8.2.1 Restriction Fragment Length Polymorphism
8.2.2 Single-Strand Conformation Polymorphism
8.2.3 Denaturing Gradient Gel Electrophoresis
8.2.4 Temporal Temperature Gradient Electrophoresis
8.2.5 Heteroduplex Analysis
8.2.6 Amplification Refractory Mutation System
8.2.7 Mismatch Cleavage
8.2.8 Truncated Protein Detection
9 Study of the Function of DNA Sequences
9.1 Study of Transcription in Tissues
9.1.1 Ribonuclease Protection Assay
9.1.2 Real-Time Quantitative PCR Assay
9.1.3 In Situ Hybridization
9.1.4 Fluorescence In Situ Hybridization on Chromosomes
9.1.5 In Situ Polymerase Chain Reaction
9.1.6 Microarrays
9.2 Mutation
9.3 In Vitro Translation
9.4 Expression Systems
9.4.1 Bacterial expression systems
9.4.2 Baculovirus expression systems
9.4.3 Other expression systems
9.4.4 Heterologous expression systems in mammalian cells
9.4.5 Transfection methods
9.4.6 Multigene co-transfection
9.4.7 Transient and stable transfection
9.4.8 Reporter genes
9.5 Transgenic mice
9.5.1 Transgenic methods
9.6 Regulation of transgenic expression
9.6.1 Tetracycline gene expression regulation system
9.6.2 Ecdysone gene expression regulation system
9.7 Gene therapy
9.8 Genomics
10 Computer applications
10.1 Important considerations
10.2 Problems in practice
11 Career planning advice: a short Machiavellian course for young researchers
12 Concluding remarks
Appendix 1
Icons
Standard solutions and bacterial culture media
Solutions
Bacterial culture media
Glossary
Appendix 2
Suppliers
Recommended literature
Casual reading
Index
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There's no electronic version?????.
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5. Protein Biochemistry and Proteomics Click to view larger image Author: (Germany) Rehm, ed.
Publisher: Science Press
Publication date: 2007-1-1 Word count: 350000 Edition: 1 Page count: 236 Printing date: 2007/01/01 Format: Impression: Paper: Offset paper ISBN: 9787030182180 Binding: Hardcover Category: Books >> Natural Sciences >> Biological Sciences >> Biophysics
Customer rating: 2 customer reviews in total View review summary Content summary
In the lab, have you ever followed the standard procedure strictly yet ended up with nothing? Do you want to improve your work? Do you want to learn more about the various methods in a certain research field? Perhaps the "Experimenter" series will be of help to you.
This book is one of the "Experimenter" series, and from the viewpoint of authoritative figures, it tells you about new methods in protein biochemistry and proteomics research. You can fully appreciate the excitement and frustration the authors have experienced from the challenges of the laboratory. With these tips and tricks, your experiments will have a higher chance of success. But this valuable laboratory manual is by no means just a collection of methods:
It points out the way out of experimental dilemmas for you, and cultivates your intuition for choosing the right experiment at the right time.
It concisely summarizes the steps of routine methods (such as column chromatography and gel electrophoresis), and also lists the advantages and disadvantages of different methods.
It describes in detail the advances in ligand binding experiments, antibody production and microsequence analysis.
It discusses some special methods, such as solubilization and reconstitution of membrane proteins, glycoprotein analysis, or determination of the subunits of oligomeric proteins.
One section provides an introductory explanation of proteomics.
Moreover, like other books in this system, when you begin to doubt yourself and do not know why you are doing all this, this book, with inspiring frankness, guides you out of the "dark night of the soul."
This book is suitable for reference use by teachers, graduate students, and researchers in laboratories of relevant departments in research institutes and universities in life science-related fields such as biochemistry and molecular biology, genetics, functional genomics, protein biochemistry, and proteomics, as well as R&D personnel and decision-makers in biotechnology companies. Contents
Preface
Preface to the fourth edition
Acknowledgments
Abbreviations
1 Routine techniques
1.1 Preparation of buffers
1.2 Protein quantification
1.3 Gel electrophoresis
1.4 Gel staining
1.5 Precipitation and concentration
1.6 Blotting
1.7 Autoradiography of gels and blots
2 Ligand binding
2.1 Radioligand labeling
2.2 Binding
2.3 Analysis of binding data
2.4 Ligand crosslinking
2.5 Uses of binding proteins
3 Solubilization of membrane proteins
3.1 Detergents
3.2 Solubilization
4 Detection of proteins by functional assays
4.1 Transporters
4.2 Reconstitution and reduction of membrane proteins
4.3 Flux experiments
4.4 Some constructive suggestions
5 Removal of impurities and purification
5.1 Pure entertainment - the process of protein purification
5.2 Routine purification methods
5.3 Affinity chromatography
5.4 Purity testing
5.5 Uses of purified proteins
6 Antibodies
6.1 Obtaining polyclonal antibodies
6.2 Immunoprecipitation
6.3 Immunoaffinity chromatography
6.4 Antibodies against impure proteins
6.5 Immunological detection techniques
7 Proteomics
7.1 Introduction
7.2 Sampling
7.3 Two-dimensional gel electrophoresis
7.4 Mass spectrometry of peptides and proteins
7.5 Protein chips
7.6 Microsequence analysis
7.7 Strategic perspectives
8 Subunits
8.1 Number and ratio of subunits
8.2 What force holds us together? - Binding between subunits
9 Glycoproteins
9.1 Modes, sites and roles of protein glycosylation
……
10 Gathering treasure: writing a paper
11 Desert expedition: literature research
Appendix A Professional resources
Index
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That's an eye-opener! That really broadened my horizons!
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6. Immunology Click to view larger image Author: (Germany) Luttmann, W. et al., eds.
Publisher: Science Press
Publication date: 2007-1-1 Word count: 409000 Edition: 1 Page count: 243 Printing date: 2007/01/01 Format: Impression: Paper: Offset paper ISBN: 9787030182197 Binding: Hardcover Category: Books >> Medicine >> Basic Medicine
0 customer reviews in total View review summary Content summary
This book is one of the "Experimenter" series. The book is divided into ten chapters in total, comprehensively discussing antibodies, the sources of antibodies, their effects in the whole body, and their important role as immunological tools. This book introduces flow cytometry, immunoblotting, ELISA and related immunological tests, all the way to cell separation techniques and in situ immunolocalization, and also points out to readers some issues in biostatistics that require attention. Like other books in the Experimenter series, this book focuses on explaining various modern methods in an easy-to-understand way, and discusses the advantages and disadvantages of each method.
Because immunology and related disciplines are developing very rapidly, although the experimental methods introduced in this book are not all the newest, and it does not cover every field of today's immunology, the methods introduced in this book are all the most practical and reliable. It not only explains the principles of the methods, but also introduces the experimental steps in detail, analyzes the reasons for success or failure in experiments, and compares them with other methods. In particular, it uses "tips" to remind experimenters of possible problems in experiments, how to solve them, and how to protect the operator's safety, etc. This book also provides some useful charts, background information, a glossary, a modern CD nomenclature table, and related product information, which help immunologists in their laboratory work.
This book will have considerable reference value for students, teachers, and researchers engaged in research in various fields of basic medicine, clinical medicine, and biology related to immunology. Contents
Preface
Appendix
Abbreviations
1 Antibodies
1.1 Characteristics of Antibodies
1.2 Antibody Preparation
1.3 Antibody Purification
1.4 Chemical Cross-linking and Labeling of Antibodies
2 Cell Separation
2.1 Separation Based on Cell Size and Density: Centrifugation Techniques
2.2 Separation by Means of Specific Cell Surface Molecules
3 Flow Cytometry
3.1 Principles of Operation
3.2 Fluorescent Substances
3.3 Sample Preparation
3.4 Starting Up the Flow Cytometer
3.5 Compensation and Measurement
3.6 Analysis
3.7 Modes and Configurations
4 Quantitative Immunoassays
4.1 Assay Principles
4.2 Radioimmunoassay (RIA)
4.3 Enzyme-Linked Immunosorbent Assay (ELISA)
4.4 Enzyme-Linked Immunospot Assay (ELISPOT Assay)
4.5 Microparticle Immunoassay (PIA)
4.6 Amplification Series
5 Immunoblotting
5.1 Sample Preparation
5.2 Separation of Proteins by Gel Electrophoresis
5.3 Transfer of Proteins to Membranes (Blotting)
5.4 Protein Determination
5.5 Dot and Slot Blotting
6 In Situ Immunolocalization
7 Immunoprecipitation
8 Cell Survival, Phagocytosis, Death
9 Special Immunological Assays
10 Statistics of Experimental Error
Appendix CD Antigens
Glossary (Specialized Terminology)
Index
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These books are not cheap, and posting electronic versions here could violate copyright law. Besides, molecular biology is just one of the research tools in nutrition science, and the principles are all interconnected. Just pick one book.
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7. Cell Biology Experiment Handbook (Third Edition)Volume 1Author: Celis, J. (Denmark), Editor-in-Chief
Publisher: Science Press
Publication Date: 2008-1-1 Word Count: 1115000 Edition: 1 Page Count: 586 Printing Date: 2008/01/01 Format: 16mo Printing Run: 1 Paper: Offset Paper ISBN: 9787030203830 Binding: Hardcover Category: Books >> Natural Sciences >> Biological Sciences >> Cytology
Total customer reviews: 0 View review summary Editorial Recommendations
As with the second edition of this book, this edition is also divided into four volumes. Volume 1 covers tissue culture and related techniques, viruses, antibodies, and immunocytochemistry. Volume 2 covers organelles and cell structure, as well as cell biology detection techniques. Volume 3 deals with imaging techniques, electron microscopy, scanning probe and scanning electron microscopy, microdissection, tissue arrays, cytogenetics and in situ hybridization, genomics, transgenics, gene knockout and gene knockdown methods, etc. The final volume covers macromolecule transfer, expression systems, and gene expression models in addition to various proteomics techniques. The appendix collects representative cultured cell lines and their characteristics, cell biology internet resources, and bioinformatics resources in computer-simulated proteome analysis systems. This handbook uniquely provides the classic and latest techniques indispensable for life science research. If you lack experts around you, this handbook can help you at any stage of your research career to use various techniques and model systems to study biological questions. The techniques introduced in this book are presented in a user-friendly, step-by-step manner, and it also teaches you some useful tips to avoid minor troubles that may be encountered in experimental operations.
Book Features:
An indispensable tool book and experimental companion for biology laboratories;
Straightforward and easy-to-follow protocols, step-by-step procedures, user-friendly layout design;
Tracks cutting-edge technologies, provides the latest information to solve complex biological problems;
Uses a large number of illustrations, some in full color, to demonstrate operational steps and results;
Compiled with contributions from more than 500 first-class researchers with hands-on experience worldwide.
Guided Reading Edition:
The preface and table of contents of this book have been translated into Chinese, the main text retains the original English version, and a Chinese guided reading article written by Professor Zhang Jingbo, doctoral supervisor at the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, is included. Content Overview
This handbook covers classic cell biology experimental protocols and has important guiding significance for life science research. The updated third edition includes 165 new articles, covering both classic and the latest experimental techniques. Each technique is presented step by step in a user-friendly format, with introductions to some practical tips and common errors. Important experimental steps and results are accompanied by illustrations for ease of understanding and use. The compilation and publication of this book enables researchers at different levels to use corresponding techniques and system models to solve basic biological problems during their research.
Volume 1: Tissue and cell culture related techniques, viruses, antibodies, immunocytochemistry.
Volume 2: Organelles and cell structure, as well as cell biology detection techniques.
Volume 3: Imaging techniques, microscopy techniques, tissue arrays, cytogenetics and in situ hybridization, genetic engineering and genomics.
Volume 4: Macromolecule transfer, expression systems, gene expression models, proteins. Table of Contents
Volume 1 Table of Contents
Other Volume Tables of Contents
Contributors
Preface
A Cell and Tissue Culture: Related Techniques
Part 1 General Techniques
1. Establishing a Cell Culture Laboratory
2. General Procedures for Cell Culture
3. Cell Counting
4. Cell Proliferation Assays: Various Improved Methods for Determining Cultured Cell Numbers
5. Development of Serum-Free Media: Optimization of Nutritional Components and Delivery Methods
6. Cell Line Identification
7. Detection of Microbial and Viral Contamination in Cell Lines
Part 2 Culture of Special Cell Types: Stem Cells
8. Neural Crest Stem Cells
9. Primary Animal Skeletal Stem Cells: Methods for Isolating and Analyzing Bone Marrow Stromal Cells BMSCs from Primary Mouse and Human Bone Marrow
10. Establishing Embryonic Stem Cell Lines from Adult Somatic Cells by Nuclear Transfer
11. Isolation and In Vitro Proliferation of T Cells
12. Generation of Human and Mouse Dendritic Cells
Part 3 Culture of Special Cell Types: Hematopoietic Cells, Mesenchymal Cells and Epithelial Cells
13. Basic In Vitro Clonal Culture of Hematopoietic Cells in Semi-Solid Agar Culture
14. Human Skeletal Muscle Cells
15. Growth of Canine Kidney Cells and Their Application in Epithelial Cell Biology Research
16. Culture of Human Epidermal Keratinocytes and Retroviral Infection
17. Three-Dimensional Culture of Normal and Malignant Human Mammary Epithelial Cells to Maintain In Vivo-Like Architecture
18. Primary Culture of Drosophila Embryonic Cells
19. Laboratory Culture of Nematodes and Other Non-Symbiotic Nematodes
Part 4 Somatic Cell Differentiation and Reprogramming
Part 5 Immortality
Part 6 Somatic Cell Hybridization
Part 7 Cell Separation Techniques
Part 8 Cell Cycle Analysis
Part 9 Cytotoxicity and Cell Growth Assays
Part 10 Apoptosis
Part 11 Cell Transformation, Tumorigenicity, Invasion and Wound Healing Assays
Part 12 Electrophysiological Methods
Part 13 Organ Culture
Part 14 Virus Growth and Purification
C Antibodies
Part 15 Production and Purification of Antibodies
D Immunocytochemistry
Part 16 Immunofluorescence
E Appendix
Volume 2Preface
A Organelles and Cell Structure
Part 1 Isolation: Plasma Membrane, Organelles, Cell Structures
1 Detergent-Resistant Membranes and Cholesterol Removal
2 Isolation and Subfractionation of Plasma Membranes, Purification of Caveolae from Lipid Rafts
3 Immunoisolation of Organelles Using Magnetic Solid-Phase Supports
4 Isolation of the Golgi Complex from Rat Liver
5 Isolation of Structural Domains of Rough and Smooth Membranes of Rat Liver Endoplasmic Reticulum
6 Purification of COPI Vesicles
7 Purification of Clathrin-Coated Vesicles from Midbrain, Liver and Adrenal Gland
8 Isolation of Phagosomes Containing Latex Beads and Mycobacteria
9 Isolation of Peroxisomes
10 Isolation of Pure Mitochondria from Mammalian Tissues and Cultured Cells
11 Subcellular Fractionation and Isolation of Dense-Core Secretory Granules from Neuroendocrine Cell Lines Using [S] Sulfate Metabolic Labeling
12 Preparation of Synaptic Vesicles from Mammalian Brain
13 Preparation of Proteasomes
14 Preparation of Cilia from Human Tracheal Epithelial Cells
15 Isolation of Nucleoli
16 Purification of Spliceosomes Containing Intermediates
17 Isolation of Cajal Bodies
18 Replication Clusters: Labeling Strategies for Chromosome Building and Chromatin Dynamics Analysis
19 Chromosome Isolation for Flow Analysis and Sorting
Part 2 Live Staining of Cells/Organelles
20 Live Staining of Cells with Fluorescent Lipids
21 Labeling Endocytic Vesicles with Fluorescent Probes to Study Fluid-Phase Endocytosis
Part 3 Protein Purification
22 Preparation of Porcine Brain Tubulin
23 Purification of Smooth Muscle Actin
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B Assay Methods
Part 4 Endocytic and Exocytic Pathways
Part 5 Membranes
Part 6 Pure Materials
Part 7 Nuclear Transport
Part 8 Chromatin Assembly
Part 9 Signal Transduction Assays
Part 10 Detection and Models of Motility In Vitro and In Vivo
Part 11 Mechanical Stress in Single Cells
C AppendixVolume 3
Preface
A Imaging Techniques
Part 1 Optical Microscopy
1. Fluorescence Microscopy
2. Total Internal Reflection Fluorescence Microscopy
3. Frequency Limitations in Microscopy and Appropriate Sampling
4. Optical Tweezers: Applications in Dynein Research
Part 2 Digital Image Microscopy
5. How to Acquire Electronic Images in Optical Microscopic Observation of Biological Specimens - Introduction
6. Image Contrast Enhancement Microscopy
Part 3 Confocal Microscopy of Living and Fixed Cells
7. Spinning Disk Confocal Microscopy of Living Cells
8. Confocal Microscopy of Drosophila Embryos
9. Application of UV Laser Microbeams in Drosophila Embryo Dissection
Part 4 Fluorescence Microscopy of Living Cells
10. Introduction to Live-Cell Fluorescence Imaging: Item-by-Item Annotations
11. Cytoskeletal Proteins
12. Systematic Cellular Localization of Novel Proteins
13. Single-Molecule Imaging in Living Cells by Total Internal Reflection Fluorescence Microscopy
14. Live-Cell Fluorescence Speckle Microscopy of Actin Cytoskeleton Dynamics and Drug Perfusion Interference
15. Imaging Fluorescence Resonance Energy Transfer Between Green Fluorescent Protein Variants in Living Cells
Part 5 Application of Fluorescent Dyes to Study Physical Parameters in Cells
16. Determination of pH in Living Cell Endosomes Using Dual-Excitation Fluorescence Imaging
17. Genome-Wide Screening of Protein Localization in Fission Yeast Cells
18. Large-Scale Protein Localization in Yeast
Part 6 Digital Imaging Production, Analysis, Storage and Presentation
19. Dehazing: Reconstructing Images with Deconvolution Techniques
20. Overview of Techniques in Biological Imaging Analysis
21. Imaging Data in Biological Imaging Databases, A Biologist's Imaging Database
B Electron Microscopy
Part 7 Specimen Preparation Techniques
22. Fixation and Embedding of Cells and Tissues for Transmission Electron Microscopy
23. Negative Staining Techniques
24. Glycerol Spray/Low-Angle Rotary Metal Shadowing
Part 8 Cryotechniques
25. Rapid Freezing of Biological Specimens for Freeze-Fracturing and Deep Etching
26. Freeze-Fracturing and Freeze-Etching
Part 9 Electron Microscopy of the Cytoskeleton
27. Electron Microscopy of Extracted Cytoskeletons: Negative Staining, Cryo-Electron Microscopy, and Correlation with Light Microscopy
28. Correlation of Light and Electron Microscopy of the Cytoskeleton
Part 10 Immunoelectron Microscopy
29. Immunoelectron Microscopy with Lowicryl Resin
30. Immunocytochemistry of Ultrathin Frozen and Plastic Sections
31. Direct Immunogold Labeling of Protein Complex Components
C Scanning Probe and Scanning Electron Microscopy
Part 11 Scanning Probe and Scanning Electron Microscopy
32. Atomic Force Microscopy in Biological Research
33. Field Emission Scanning Electron Microscopy and Observation of the Cell Interior
D Microdissection
Part 12 Microdissection of Tissues and Chromosomes
34. Laser Capture Microdissection
35. Chromosome Microdissection Using Conventional Methods
36. Micromanipulation of Chromosomes and the Mitotic Spindle Using Laser Microsurgery (Laser Scissors) and Laser-Generated Optical Forces (Laser Tweezers)
E Tissue Arrays
Part 13 Tissue Arrays
37. Tissue Microarrays
F Cytogenetics and In Situ Hybridization
Part 14 Cytogenetics
38. Basic Cytogenetic Techniques: Culture, Slide Preparation and G-Banding
39. A Common and Reliable Method for Obtaining High-Yield Metaphase Chromosome Specimens from Adherently Growing Cell Lines: Rapid Confirmation of Cellular Chromosome Composition
Part 15 In Situ Hybridization
40. Mapping Cloned DNA on Metaphase Chromosomes by Fluorescence In Situ Hybridization
41. Breakpoint Mapping Using Human Genome Project Resources
42. Fine Mapping of Gene Order Using Extended Chromatin Methods
43. In Situ Hybridization for mRNA Species in Cultured Cells
44. Simultaneous Detection of DNA, RNA and Protein by In Situ Hybridization
45. Fluorescence Observation of Genome Structure and DNA Replication at the Single-Molecule Level
G Genomics
Part 16 Genomics
46. Genomic DNA Microarrays for Comparative Genomic Hybridization
47. Single Nucleotide Polymorphism Genotyping Using Tag Arrays and Microsequencing
48. Single Nucleotide Polymorphism Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
49. Single Nucleotide Polymorphism Analysis Using ZipCode Tag Microsphere Technology
50. PCR Amplification Methods for Preserving Quantitative Differences Between Two Complex Genomes
H Methods for Transgenesis, Gene Knockout and Gene Knockdown
Part 17 Methods for Transgenesis, Gene Knockout and Gene Knockdown
51. Production of Transgenic Mice by Pronuclear Microinjection
52. Gene Targeting by Homologous Recombination in Embryonic Stem Cells
53. Conditional Gene Knockout: The Cre-lox System
54. RNAi-Mediated Gene Silencing in Mammalian Cells
55. Antisense Oligonucleotides
Volume 4Table of Contents Other Tables of Contents
Contributors
Preface
A Transfer of Macromolecules
Part 1 Proteins
Part 2 Genes
Part 3 Somatic Cell Nuclear Transfer
B Expression Systems
Part 4 Expression Systems
C Gene Expression Models
Part 5 Differential Gene Expression
D Proteins
Part 6 Protein Identification and Analysis
Part 7 Specimen Fractionation for Proteomics
Part 8 Gel Electrophoresis
Part 9 Detection of Proteins in Gels
Part 10 Gel Pattern Analysis of Post-Translationally Modified Proteins
Part 11 Protein/Protein and Protein/Small Molecule Interactions
Part 12 Omics of Functional Proteins
Part 13 Protein/DNA Interactions
Part 14 Protein Degradation
Part 15 Mass Spectrometry: Protein Identification and Interactions
E
Part 16 Appendix
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Publisher: People's Medical Publishing House
Publication time: 2006-7-1 Word count: 469000 Edition: 1 Page count: 251 Printing time: 2006/07/01 ISBN: 9787117077705 Content introduction
The book is divided into 5 chapters. Chapter 1 briefly introduces the concept of gene expression and the basic theory of gene expression regulation. Because the regulation of gene expression by nutrients involves these basic theories of molecular biology, introducing them at the beginning of the book helps lay a foundation for learning the knowledge that follows. Chapter 2 covers the regulation of gene expression by nutrients and their effects on genome structure and stability, which is one of the main research areas of molecular nutrition. In Chapter 3, using various nutrients as examples, the regulation of gene expression by each nutrient is introduced in detail, because although nutrient regulation of gene expression has some common features, each also has its own characteristics. Chapter 4 covers the effects of gene polymorphism on nutrient absorption, metabolism and utilization, which is another main research area of molecular nutrition. Chapter 5, on the basis of the above chapters, explores the patterns and mechanisms of the effects of nutrient-gene interactions on health, namely the role of nutrient-gene interactions in the occurrence of disease. This book contains a great deal of content and involves much knowledge of molecular biology. After the main text, major reference materials are attached, which can be used by students for further reading and by teachers for reference in teaching. An English-Chinese index is also attached at the end of the book for lookup. This book can be used as a textbook for undergraduate and graduate students in nutrition and food majors. Table of contents Introduction
Chapter 1 The concept of gene expression and the basic theory of gene expression regulation
Section 1 Basic concepts of gene expression
Section 2 Basic theory of gene expression regulation
Section 3 The significance of gene expression regulation
Chapter 2 Regulation of gene expression by nutrients and effects on genome structure and stability
Section 1 Regulation of gene expression by nutrients
Section 2 Effects of nutrients on genome structure and stability
Chapter 3 Regulation of gene expression by various nutrients
Section 1 Regulation of gene expression by protein
Section 2 Regulation of gene expression by fat
Section 3 Regulation of gene expression by carbohydrates
Section 4 Regulation of gene expression by calcium
Section 5 Regulation of gene expression by iron
Section 6 Regulation of gene expression by zinc
Section 7 Regulation of gene expression by selenium
Section 8 Regulation of gene expression by chromium
Section 9 Regulation of gene expression by vitamin A
Section 10 Regulation of gene expression by vitamin D
Section 11 Regulation of gene expression by vitamin E
Section 12 Regulation of gene expression by biotin
Chapter 4 Effects of gene polymorphism on nutrient absorption, metabolism and utilization
Section 1 Effects of gene polymorphism on folate metabolism
Section 2 Effects of vitamin D receptor gene polymorphism on calcium metabolism
Section 3 Effects of gene polymorphism on cholesterol metabolism
Section 4 Effects of gene polymorphism on iron metabolism
Section 5 Gene polymorphism and lactose intolerance
Chapter 5 The role of nutrient-gene interactions in the occurrence of disease
Section 1 Overview
Section 2 Effects of early-life nutrition on obesity-related diseases in adulthood
Section 3 Effects of nutrition-gene interactions on hyperlipidemia
Section 4 Effects of nutrient-gene interactions on the occurrence of obesity
Section 5 Effects of nutrient-gene interactions on salt-sensitive hypertension
Section 6 Effects of nutrient-gene interactions on the occurrence of diabetes
References
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2. Molecular Nutrition Author: (US) J. Zempleni, (Germany) H. Daniel, edited, translated by Luo Xugang et al.
Publisher: Science Press
Publication time: 2008-5-1 Word count: 614000 Edition: 1 Page count: 414 Printing time: 2008/05/01 Format: Large 16 mo Printing count: 1 Paper: Offset paper ISBN: 9787030173713 Content introduction
Molecular nutrition is the fastest-growing science in the field of nutrition. This book is currently the only Chinese translation published in China on research progress in the field of molecular nutrition. The book reviews the latest and most important research results on molecular nutrition published by first-class international experts. The book is divided into 24 chapters, describing in detail the research methods of molecular nutrition, the dynamic homeostasis of nutrients in cells, cell proliferation and apoptosis, signal transduction, gene expression and proteolysis, the physiological roles of nucleic acids and events at the molecular level, and the molecular mechanisms of genetically modified foods and food allergies.
This book is faithful to the original text and reflects the style and flavor of the original book to the greatest extent. It can be used as a reference book for teaching and research personnel and graduate students in nutrition, animal nutrition, biochemistry, molecular biology and pharmaceutical sciences. Table of contents
Translator's preface
Foreword
Part 1 Methods of molecular nutrition research
1 Genome and post-genome
Ji Zhang
2 Prospects for post-genomic nutrition research
Hannelore Daniel
Part 2 Cellular nutrient homeostasis, cell proliferation and apoptosis
3 Molecular physiology of plasma membrane transport proteins for organic nutrients
Hannelore Daniel
4 Intracellular transport and compartmentalization of vitamins and their physiologically active forms
Donald B.McCormick
5 Nutrient homeostasis in proliferating cells
Janos Zempleni
6 Nutrition and apoptosis
John C.Mathers
Part 3 Roles of nutrients in signal transduction, gene expression and proteolysis
7 Regulation of gene expression by glucose in mammals
Fabienne Foufelle and Pascal Ferre
8 Amino acid-dependent transcriptional regulation in NgL animal cells
Michael S. Kilberg, Van Leung-Pineda and Chin Chen
9 Fatty acids and gene expression
Ulrike Beisiegel, Joerg Heeren and Frank Schnieders
10 The role of retinoic acid receptors and retinoid X receptors in the molecular mechanism regulating the action of vitamin A
Dianne R. Soprano and Kenneth J. Soprano
11 Regulation of gene expression by biotin, vitamin B6 and vitamin C
Krishnamurti Dakshinamurti
12 Selenium and vitamin E
Alexandra Fischer, Josef Pallauf, Jonathan Maj ewicz, Anne Marie Minihane and Gerald Rimbach
13 Sphingolipids: new strategies for cancer treatment and prevention
Eva M.Schmelz
14 Health-promoting effects of isoflavones in food
Thomas M. Badger, Martin J. J. Ronis and Nianbai Fang
15 Ubiquitination and proteasome-dependent proteolytic mechanisms in skeletal muscle
Didier AttaiX, Lydie Combarct, Anthony J. Kee and Daniel Taulandier
Part 4 Nucleic acids and nucleic acid-binding compounds
16 Diet, DNA methylation and cancer
Judith K.Christman
17 Biotinylation of histones in human cells
Janos Zempleni
18 Niacin nutritional status, poly(ADP-ribose) metabolism and genome instability
Jennifer C. Spronck and James B. Kirkland
Part 5 Effects of molecular activity on physiology
19 Assembly of plasma lipoproteins that transport triglycerides
Joan A.Higgins
20 Regulation of cellular cholesterol
Ji-Young Lee, Susan H. Mitmesser and Timothy P. Carr
21 Year 2002: Assessment of the effect of nutrition on the incidence of cataracts
Allen Tavlor and Mark Siegal
22 Nutrition and immune function
Parveen Yaqoob and Philip C. Calder
Part 6 Food
23 Molecular mechanisms of food allergy
J. Steven Stanley and Gary A. Bannon
24 Safety evaluation of genetically modified foods
Steve L.Taylor
Index Book excerpt Illustrations
1 Genome and post-genome Ji Zhang
(1 Department of Pathology and Microbiology, University of Nebraska Medical Center; 2 Human Molecular Genetics Center, Munroe-Meyer Institute, University of Nebraska Medical Center, Omaha, Nebraska, USA)
Introduction
In recent years, tremendous progress in the field of genome research has driven the precise determination of the molecular mechanisms of human health and disease, providing great potential for improving health, reducing morbidity and mortality, and preventing disease. Understanding the molecular mechanisms by which healthy adults respond differently to nutrient intake will greatly advance the development of nutrition science, so it is quite important for nutritionists to acquire knowledge of the necessary techniques and resources for genome research. Originally, genomics referred to the scientific principles of mapping, sequencing and analyzing an organism's genome, that is, the entire set of genes and chromosomes. At present, the research focus of genomics has shifted from structural analysis of the genome (structural genomics) to functional analysis of the genome (functional genomics). The goal of structural genomics is to construct high-resolution genetic, physical and transcriptional maps of an organism and ultimately determine its entire DNA sequence. However, functional genomics represents a new stage of genome research and refers to developing innovative technologies on the basis of large amounts of structural genomics information. The first part of this chapter will focus on the tools and reagents used in structural genomics; the second part will focus on DNA microarray technology, which is the representative of today's functional genomics.
Structural genomics
Genome, genetic mapping and physical mapping
The human genome contains nearly 3 billion nucleotide base pairs and carries the genetic code for 30000 to 100000 genes. The diploid genome DNA constitutes 22 pairs of autosomes and two sex chromosomes. Each chromosome contains a linear DNA molecule, which is characterized by containing 3 functional elements that are necessary for successful chromosome replication during cell division: 1 self-replicating sequences; 2 centromeres, namely the attachment points of the spindle during mitosis or meiosis; 3 telomeres, which ensure the complete replication of the terminal chromatids. A genome map is defined as the relative positions of different loci (genes, regulatory sequences, polymorphism marker sequences, etc.) in a DNA molecule. There are two different methods for mapping genome loci, namely genetic mapping and physical mapping.
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3.Integrated Applied PhysiologyAuthor: Feng Zhiqiang, Editor-in-Chief
Publisher: People's Military Medical Press
Publication time: 2006-5-1 Word count: 540000 Edition: 1 Page count: 345 Printing time: 2006/05/01 Format: Printing count: Paper: Offset paper ISBN: 9787509102237 Content introduction
Under the guidance of materialist dialectics, this book integrates physiological knowledge with related research progress and relevant humanities knowledge to form a unique knowledge framework. The book consists of 6 chapters and 55 sections in total: the development of physiology, the basic characteristics of life, homeostasis mechanisms, cell function, the functional activities and coordination mechanisms of the body's various systems, and the body's protective mechanisms. Each section explains a relatively complex physiological issue in an integrated form, with both a certain breadth and corresponding depth; at the same time, it also focuses on combining the basic theories of physiology with certain clinical changes or research progress; on the premise of taking physiological knowledge as the core, it proposes new hypotheses or issues not yet clarified, and especially the introduction of the thoughts and spirit of some Nobel Prize winners in Physiology or Medicine has the effect of enlightening readers' thinking. This book reorganizes physiological knowledge with a novel conception, and its content is comprehensive and substantial, with highly integrated figures mainly designed by the author. This book is mainly used for graduate students and undergraduate students of various majors in medical colleges to learn physiological knowledge and conduct thinking training on complex issues, and it also has reference value for teachers engaged in basic and clinical teaching. Table of contents
Chapter 1 The development of physiology
Section 1 The development of physiology and the role of materialist dialectics in it
1. Ancient understanding of human physiology
2. The development of modern physiology
3. The development of contemporary physiology
4. The role of materialist dialectics in the development of physiology
5. Ideas that should be upheld and issues that should be noted in physiology research
Section 2 The development of physiology in China
1. Overview of the development of physiology in China
2. China's contributions to the development of physiology
3. Contributions of the older generation of physiologists to the development of physiology in China
4. The development of China's physiology cause is closely linked to the fate of the motherland
Section 3 The macro level and micro level of physiology research
1. Content of macro-level and micro-level research
II. Progress and achievements in research at the macro and micro levels
III. The complementary role of research at the macro and micro levels
Section 4 Methods and techniques in physiology research
I. Methods of physiology research
II. Intracellular recording techniques and extracellular recording techniques
III. Development of the conditions for physiology research
Chapter 2 Basic characteristics of life
Section 1 Metabolism
I. Metabolism and its two aspects
II. The relationship between metabolism and the functions of the body's various systems
Section 2 Excitability and its manifestations
I. The concept of excitability
II. The manifestations of excitability
Section 3 The origin and movement of life
I. The origin of life
II. Individual growth and development and its regulation
III. The meaning of life and attitudes toward life
Section 4 Information transmission in life activities
I. Major events in the study of cellular information transmission
II. Informational substances in the body and their movement
III. The role and mechanisms of transmembrane information transmission
IV. Information transmission in life activities and its characteristics
Section 5 Coordination between the body and its environment and its mechanisms
Chapter 3 Homeostatic mechanisms
Section 1 Regulation of the body's functional activities and automatic control
Section 2 The physicochemical properties of intracellular fluid and extracellular fluid and the mechanisms that stabilize them
Section 3 Acid-base balance in body fluids and the mechanisms that maintain it
Section 4 The stabilization mechanism of plasma osmotic pressure and changes in body fluid osmotic pressure
Section 5 The stabilization mechanism of the body's blood pressure
Section 6 Absorption and excretion functions and their role in homeostasis
Section 7 The stabilization mechanism of the body's body temperature
Chapter 4 Cell functions
Section 1 Active transport and passive transport functions of the cell membrane
Section 2 Ion channels and membrane currents in the cell membrane
Section 3 Activity changes in nerve cells and their mechanisms
Section 4 The process and mechanisms of synaptic transmission
Section 5 The motor function of muscle cells
Section 6 The function, production and destruction of red blood cells
Section 7 Electrical activity and physiological properties of cardiac autorhythmic cells and non-autorhythmic cells
Section 8 The role of ions in the activity of ventricular myocardial cells
Chapter 5 The functional activities of the body's various systems and their coordination mechanisms
Section 1 The heart's blood-pumping function and its changes
Section 2 The formation mechanism of arterial blood pressure
Section 3 The functional activity and regulation of microcirculation
Section 4 Regulation of cardiovascular activity
Section 5 The formation and regulation of respiratory movement
Section 6 Gas exchange and transport
Section 7 Digestive function and its coordination
Section 8 The mechanisms underlying hunger and satiety
Section 9 The movement and regulation of energy substances in the body
Section 10 The process of urine formation and excretion and its regulation
Section 11 Regulation of the body's autonomic functional activities
Section 12 The process and mechanisms of vision
Section 13 The process and mechanisms of hearing
Section 14 Pain and analgesia
Section 15 Other sensory functions of the body
Section 16 Changes in the body's functional activities during exercise and their regulation
Section 17 Stretch reflex and reverse stretch reflex
Section 18 The mechanisms maintaining the body's posture and movement
Section 19 Wakefulness and sleep
Section 20 Memory and forgetting
Section 21 Unconditioned reflexes and conditioned reflexes
Section 22 Regulation of the body's functional activities by the cerebral cortex
Section 23 The effects and regulation of endocrine and exocrine secretion
Section 24 The effects and mechanisms of hormones on receptor regulation
Section 25 Synergistic and antagonistic effects of hormones
Section 26 The effects of sex hormones and the regulation of their secretion
Chapter 6 The body's protective mechanisms
Section 1 The body's immune function
Section 2 Blood coagulation and anticoagulation mechanisms
Section 3 Damaging factors in the stomach and the stomach's self-protective effects
Section 4 The body's response to emergencies and its mechanisms
Section 5 Damaging factors in the environment and the body's protective mechanisms
References
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These books are all good, especially the third one, "Integrative Physiology," which is extremely helpful for a comprehensive understanding of the animal body!
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4.Molecular Biology and GenomicsAuthor: Edited by (Germany) Muelhardt
Publisher: Science Press
Publication time: 2007-7-1 Word count: 421000 Edition: 1 Page count: 257 Printing time: 2007/07/01 Format: Printing: Paper: Offset paper ISBN: 9787030193896 Binding: Hardcover Category: Books >> Natural Science >> Biological Science >> Genetics
Content introduction
At present, a major confusion faced by laboratory personnel is how to use the correct tools and methods to explain the structure, function and expression interpretation of the relevant proteins encoded by the genome. "Molecular Biology and Genomics" by Muelhardt of the University of Marburg in Germany provides everyone with many useful laboratory tips and techniques that can greatly improve the success rate and accuracy of experiments. This laboratory guide briefly and clearly introduces the various modern methods and various routine methods involved in molecular biology and genomics, and discusses the advantages and disadvantages of each method.
As one of the "Experimenter" series, this book adheres to the consistent style of the series and remains a very practical laboratory manual. In a concise and clear style, using more than 100 charts, it provides a large number of practical tips for the broad community of molecular biology and genomics laboratory personnel to supplement and improve basic knowledge, thereby greatly improving the accuracy and success rate of experiments.
This book is suitable for laboratory personnel, scientific researchers, teachers, senior undergraduates and graduate students in the fields of molecular biology and genomics. Contents
Introduction
Preface to the first edition
Acknowledgments
1 What is molecular biology?
1.1 Basic knowledge of molecular biology: introducing beginners to the molecular world
1.2 Basic requirements of molecular biology
1.3 Laboratory safety
2 Introduction to basic methods
2.1 Differences in nucleic acids
2.2 Concentration of nucleic acids by precipitation
2.2.1 Ethanol precipitation method
2.2.2 Concentration devices
2.2.3 Freeze drying
2.2.4 Salting out
2.3 Nucleic acid purification
2.3.1 Phenol-chloroform extraction purification method
2.3.2 Polyethylene glycol precipitation method
2.3.3 Protein-binding membrane purification method
2.3.4 Anion exchange column purification method
2.3.5 Glass milk purification method
2.3.6 Cesium chloride density gradient centrifugation method
2.3.7 Dialysis method
2.4 Determination of nucleic acid concentration
2.4.1 Spectrophotometer method
2.4.2 Agarose gel electrophoresis method
2.4.3 Dot quantification method
2.4.4 Fluorescence quantification method
2.4.5 Nucleic acid detector method
2.4.6 Enzyme labeling method
2.5 DNA preparation methods
2.5.1 Small-scale preparation of plasmid DNA
2.5.2 Large-scale preparation of plasmid DNA
2.5.3 Bacterial culture medium
2.5.4 Preparation of phage DNA
2.5.5 Preparation of single-stranded DNA using helper phage
2.5.6 Genomic DNA Preparation
3 Molecular Biology Tools
3.1 Restriction Enzymes
3.1.1 Nomenclature
3.1.2 Activity Assay
3.1.3 Restriction Enzyme Digestion Reaction
3.1.4 Difficulties in Restriction Enzyme Digestion
3.1.5 Guidelines for Restriction Enzyme Digestion
3.2 Gels
3.2.1 Agarose Gels
3.2.2 Recovery of DNA Fragments from Agarose Gels
3.2.3 Polyacrylamide Gels
3.2.4 Recovery of DNA Fragments from Polyacrylamide Gels
3.2.5 Pulsed-Field Gel Electrophoresis
3.2.6 Capillary Electrophoresis
3.3 Blotting
3.3.1 Southern Blotting
3.3.2 Northern Blotting
3.3.3 Dot Blotting and Slot Blotting
4 Polymerase Chain Reaction
4.1 Standard Polymerase Chain Reaction
4.2 Modified Polymerase Chain Reaction
4.2.1 Nested Polymerase Chain Reaction
4.2.2 Multiplex Polymerase Chain Reaction
4.2.3 Long-Range Polymerase Chain Reaction
4.3 Applications of Polymerase Chain Reaction
4.3.1 Reverse Transcription Polymerase Chain Reaction
4.3.2 Rapid Amplification of cDNA Ends
4.3.3 Amplification of Identical Products
4.3.4 Classic Quantitative Polymerase Chain Reaction
4.3.5 Real-Time Quantitative Polymerase Chain Reaction
4.3.6 Inverse Polymerase Chain Reaction
4.3.7 Biotin-RAGE Polymerase Chain Reaction
4.3.8 Modified Primer Mutagenesis
4.3.9 Amplification Refractory Mutation System
4.3.10 In Situ Polymerase Chain Reaction
4.3.11 Cycle Sequencing
4.3.12 cDNA Synthesis
4.3.13 Single-Cell Polymerase Chain Reaction
5 RNA
5.1 RNase Inactivation
5.2 RNA Extraction Methods
5.2.1 One-Step Method
5.2.2 Ethylphenyl Polyethylene Glycol Lysis Method
5.2.3 Basic Information
5.2.4 RNA Concentration Determination
5.3 mRNA Isolation Methods
5.3.1 Purchasing RNA
5.4 Reverse Transcription: cDNA Synthesis
5.5 In Vitro Transcription: RNA Synthesis
5.6 RNA Interference
6 Cloning DNA Fragments
6.1 Basic Elements of Cloning
6.1.1 Vectors
6.1.2 DNA Fragments
6.1.3 Fill-In Reaction
6.1.4 DNA Quantification
6.1.5 Ligation
6.1.6 Cloning PCR Products
6.1.7 Cloning with Recombinase Systems
6.2 Choosing Cloning Vectors
6.2.1 Plasmids
6.2.2 Bacteriophages
6.2.3 Cosmids
6.2.4 P1 Artificial Chromosomes and Bacterial Artificial Chromosomes
6.2.5 Yeast Artificial Chromosomes
6.3 Choosing Bacteria
6.4 Preparation of Competent Cells and Transformation
6.4.1 Calcium Chloride Method
6.4.2 Rubidium Chloride Method
6.4.3 TSS Method
6.4.4 Electroporation
6.4.5 Titer Determination
6.5 Issues Related to Cloning
6.6 Storage of Clones
7 Hybridization: How to Detect DNA
7.1 Preparing Probes
7.1.1 Methods for Preparing Labeled Probes
7.2 Hybridization
7.2.1 Hybridization Buffers
7.2.2 Hybridization Reaction System
7.2.3 Hybridization Temperature
7.2.4 Washing
7.3 Verification of Labeled DNA
7.3.1 Autoradiography
7.3.2 Non-Radioactive Detection Methods
7.4 Screening of Recombinant DNA Libraries
7.4.1 Preparation of DNA Library Plates
7.4.2 Membrane Transfer
7.4.3 Membrane Hybridization
7.4.4 Membrane Exposure
7.4.5 Clone Probing
7.4.6 Future Library Screening
7.5 Two-Hybrid System
8 DNA Analysis
8.1 Sequencing
8.1.1 Radioactive Sequencing
8.1.2 Non-Radioactive Sequencing and Automated Sequencing Units
8.1.3 Pocket-Sized Sequencing
8.1.4 Pyrosequencing
8.1.5 Sequencing Characteristics: Octamers
8.2 Methods for Analyzing DNA Mutations
8.2.1 Restriction Fragment Length Polymorphism
8.2.2 Single-Strand Conformation Polymorphism
8.2.3 Denaturing Gradient Gel Electrophoresis
8.2.4 Temporal Temperature Gradient Electrophoresis
8.2.5 Heteroduplex Analysis
8.2.6 Amplification Refractory Mutation System
8.2.7 Mismatch Cleavage
8.2.8 Truncated Protein Detection
9 Study of the Function of DNA Sequences
9.1 Study of Transcription in Tissues
9.1.1 Ribonuclease Protection Assay
9.1.2 Real-Time Quantitative PCR Assay
9.1.3 In Situ Hybridization
9.1.4 Fluorescence In Situ Hybridization on Chromosomes
9.1.5 In Situ Polymerase Chain Reaction
9.1.6 Microarrays
9.2 Mutation
9.3 In Vitro Translation
9.4 Expression Systems
9.4.1 Bacterial expression systems
9.4.2 Baculovirus expression systems
9.4.3 Other expression systems
9.4.4 Heterologous expression systems in mammalian cells
9.4.5 Transfection methods
9.4.6 Multigene co-transfection
9.4.7 Transient and stable transfection
9.4.8 Reporter genes
9.5 Transgenic mice
9.5.1 Transgenic methods
9.6 Regulation of transgenic expression
9.6.1 Tetracycline gene expression regulation system
9.6.2 Ecdysone gene expression regulation system
9.7 Gene therapy
9.8 Genomics
10 Computer applications
10.1 Important considerations
10.2 Problems in practice
11 Career planning advice: a short Machiavellian course for young researchers
12 Concluding remarks
Appendix 1
Icons
Standard solutions and bacterial culture media
Solutions
Bacterial culture media
Glossary
Appendix 2
Suppliers
Recommended literature
Casual reading
Index
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There's no electronic version?????.
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5. Protein Biochemistry and Proteomics Click to view larger image Author: (Germany) Rehm, ed.
Publisher: Science Press
Publication date: 2007-1-1 Word count: 350000 Edition: 1 Page count: 236 Printing date: 2007/01/01 Format: Impression: Paper: Offset paper ISBN: 9787030182180 Binding: Hardcover Category: Books >> Natural Sciences >> Biological Sciences >> Biophysics
Customer rating: 2 customer reviews in total View review summary Content summary
In the lab, have you ever followed the standard procedure strictly yet ended up with nothing? Do you want to improve your work? Do you want to learn more about the various methods in a certain research field? Perhaps the "Experimenter" series will be of help to you.
This book is one of the "Experimenter" series, and from the viewpoint of authoritative figures, it tells you about new methods in protein biochemistry and proteomics research. You can fully appreciate the excitement and frustration the authors have experienced from the challenges of the laboratory. With these tips and tricks, your experiments will have a higher chance of success. But this valuable laboratory manual is by no means just a collection of methods:
It points out the way out of experimental dilemmas for you, and cultivates your intuition for choosing the right experiment at the right time.
It concisely summarizes the steps of routine methods (such as column chromatography and gel electrophoresis), and also lists the advantages and disadvantages of different methods.
It describes in detail the advances in ligand binding experiments, antibody production and microsequence analysis.
It discusses some special methods, such as solubilization and reconstitution of membrane proteins, glycoprotein analysis, or determination of the subunits of oligomeric proteins.
One section provides an introductory explanation of proteomics.
Moreover, like other books in this system, when you begin to doubt yourself and do not know why you are doing all this, this book, with inspiring frankness, guides you out of the "dark night of the soul."
This book is suitable for reference use by teachers, graduate students, and researchers in laboratories of relevant departments in research institutes and universities in life science-related fields such as biochemistry and molecular biology, genetics, functional genomics, protein biochemistry, and proteomics, as well as R&D personnel and decision-makers in biotechnology companies. Contents
Preface
Preface to the fourth edition
Acknowledgments
Abbreviations
1 Routine techniques
1.1 Preparation of buffers
1.2 Protein quantification
1.3 Gel electrophoresis
1.4 Gel staining
1.5 Precipitation and concentration
1.6 Blotting
1.7 Autoradiography of gels and blots
2 Ligand binding
2.1 Radioligand labeling
2.2 Binding
2.3 Analysis of binding data
2.4 Ligand crosslinking
2.5 Uses of binding proteins
3 Solubilization of membrane proteins
3.1 Detergents
3.2 Solubilization
4 Detection of proteins by functional assays
4.1 Transporters
4.2 Reconstitution and reduction of membrane proteins
4.3 Flux experiments
4.4 Some constructive suggestions
5 Removal of impurities and purification
5.1 Pure entertainment - the process of protein purification
5.2 Routine purification methods
5.3 Affinity chromatography
5.4 Purity testing
5.5 Uses of purified proteins
6 Antibodies
6.1 Obtaining polyclonal antibodies
6.2 Immunoprecipitation
6.3 Immunoaffinity chromatography
6.4 Antibodies against impure proteins
6.5 Immunological detection techniques
7 Proteomics
7.1 Introduction
7.2 Sampling
7.3 Two-dimensional gel electrophoresis
7.4 Mass spectrometry of peptides and proteins
7.5 Protein chips
7.6 Microsequence analysis
7.7 Strategic perspectives
8 Subunits
8.1 Number and ratio of subunits
8.2 What force holds us together? - Binding between subunits
9 Glycoproteins
9.1 Modes, sites and roles of protein glycosylation
……
10 Gathering treasure: writing a paper
11 Desert expedition: literature research
Appendix A Professional resources
Index
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Originally posted by Roniloso on 2008-6-27 11:19 There's no electronic version?????.Sorry, there isn't one
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That's an eye-opener! That really broadened my horizons!
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6. Immunology Click to view larger image Author: (Germany) Luttmann, W. et al., eds.
Publisher: Science Press
Publication date: 2007-1-1 Word count: 409000 Edition: 1 Page count: 243 Printing date: 2007/01/01 Format: Impression: Paper: Offset paper ISBN: 9787030182197 Binding: Hardcover Category: Books >> Medicine >> Basic Medicine
0 customer reviews in total View review summary Content summary
This book is one of the "Experimenter" series. The book is divided into ten chapters in total, comprehensively discussing antibodies, the sources of antibodies, their effects in the whole body, and their important role as immunological tools. This book introduces flow cytometry, immunoblotting, ELISA and related immunological tests, all the way to cell separation techniques and in situ immunolocalization, and also points out to readers some issues in biostatistics that require attention. Like other books in the Experimenter series, this book focuses on explaining various modern methods in an easy-to-understand way, and discusses the advantages and disadvantages of each method.
Because immunology and related disciplines are developing very rapidly, although the experimental methods introduced in this book are not all the newest, and it does not cover every field of today's immunology, the methods introduced in this book are all the most practical and reliable. It not only explains the principles of the methods, but also introduces the experimental steps in detail, analyzes the reasons for success or failure in experiments, and compares them with other methods. In particular, it uses "tips" to remind experimenters of possible problems in experiments, how to solve them, and how to protect the operator's safety, etc. This book also provides some useful charts, background information, a glossary, a modern CD nomenclature table, and related product information, which help immunologists in their laboratory work.
This book will have considerable reference value for students, teachers, and researchers engaged in research in various fields of basic medicine, clinical medicine, and biology related to immunology. Contents
Preface
Appendix
Abbreviations
1 Antibodies
1.1 Characteristics of Antibodies
1.2 Antibody Preparation
1.3 Antibody Purification
1.4 Chemical Cross-linking and Labeling of Antibodies
2 Cell Separation
2.1 Separation Based on Cell Size and Density: Centrifugation Techniques
2.2 Separation by Means of Specific Cell Surface Molecules
3 Flow Cytometry
3.1 Principles of Operation
3.2 Fluorescent Substances
3.3 Sample Preparation
3.4 Starting Up the Flow Cytometer
3.5 Compensation and Measurement
3.6 Analysis
3.7 Modes and Configurations
4 Quantitative Immunoassays
4.1 Assay Principles
4.2 Radioimmunoassay (RIA)
4.3 Enzyme-Linked Immunosorbent Assay (ELISA)
4.4 Enzyme-Linked Immunospot Assay (ELISPOT Assay)
4.5 Microparticle Immunoassay (PIA)
4.6 Amplification Series
5 Immunoblotting
5.1 Sample Preparation
5.2 Separation of Proteins by Gel Electrophoresis
5.3 Transfer of Proteins to Membranes (Blotting)
5.4 Protein Determination
5.5 Dot and Slot Blotting
6 In Situ Immunolocalization
7 Immunoprecipitation
8 Cell Survival, Phagocytosis, Death
9 Special Immunological Assays
10 Statistics of Experimental Error
Appendix CD Antigens
Glossary (Specialized Terminology)
Index
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These books are not cheap, and posting electronic versions here could violate copyright law. Besides, molecular biology is just one of the research tools in nutrition science, and the principles are all interconnected. Just pick one book.
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7. Cell Biology Experiment Handbook (Third Edition)Volume 1Author: Celis, J. (Denmark), Editor-in-Chief
Publisher: Science Press
Publication Date: 2008-1-1 Word Count: 1115000 Edition: 1 Page Count: 586 Printing Date: 2008/01/01 Format: 16mo Printing Run: 1 Paper: Offset Paper ISBN: 9787030203830 Binding: Hardcover Category: Books >> Natural Sciences >> Biological Sciences >> Cytology
Total customer reviews: 0 View review summary Editorial Recommendations
As with the second edition of this book, this edition is also divided into four volumes. Volume 1 covers tissue culture and related techniques, viruses, antibodies, and immunocytochemistry. Volume 2 covers organelles and cell structure, as well as cell biology detection techniques. Volume 3 deals with imaging techniques, electron microscopy, scanning probe and scanning electron microscopy, microdissection, tissue arrays, cytogenetics and in situ hybridization, genomics, transgenics, gene knockout and gene knockdown methods, etc. The final volume covers macromolecule transfer, expression systems, and gene expression models in addition to various proteomics techniques. The appendix collects representative cultured cell lines and their characteristics, cell biology internet resources, and bioinformatics resources in computer-simulated proteome analysis systems. This handbook uniquely provides the classic and latest techniques indispensable for life science research. If you lack experts around you, this handbook can help you at any stage of your research career to use various techniques and model systems to study biological questions. The techniques introduced in this book are presented in a user-friendly, step-by-step manner, and it also teaches you some useful tips to avoid minor troubles that may be encountered in experimental operations.
Book Features:
An indispensable tool book and experimental companion for biology laboratories;
Straightforward and easy-to-follow protocols, step-by-step procedures, user-friendly layout design;
Tracks cutting-edge technologies, provides the latest information to solve complex biological problems;
Uses a large number of illustrations, some in full color, to demonstrate operational steps and results;
Compiled with contributions from more than 500 first-class researchers with hands-on experience worldwide.
Guided Reading Edition:
The preface and table of contents of this book have been translated into Chinese, the main text retains the original English version, and a Chinese guided reading article written by Professor Zhang Jingbo, doctoral supervisor at the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, is included. Content Overview
This handbook covers classic cell biology experimental protocols and has important guiding significance for life science research. The updated third edition includes 165 new articles, covering both classic and the latest experimental techniques. Each technique is presented step by step in a user-friendly format, with introductions to some practical tips and common errors. Important experimental steps and results are accompanied by illustrations for ease of understanding and use. The compilation and publication of this book enables researchers at different levels to use corresponding techniques and system models to solve basic biological problems during their research.
Volume 1: Tissue and cell culture related techniques, viruses, antibodies, immunocytochemistry.
Volume 2: Organelles and cell structure, as well as cell biology detection techniques.
Volume 3: Imaging techniques, microscopy techniques, tissue arrays, cytogenetics and in situ hybridization, genetic engineering and genomics.
Volume 4: Macromolecule transfer, expression systems, gene expression models, proteins. Table of Contents
Volume 1 Table of Contents
Other Volume Tables of Contents
Contributors
Preface
A Cell and Tissue Culture: Related Techniques
Part 1 General Techniques
1. Establishing a Cell Culture Laboratory
2. General Procedures for Cell Culture
3. Cell Counting
4. Cell Proliferation Assays: Various Improved Methods for Determining Cultured Cell Numbers
5. Development of Serum-Free Media: Optimization of Nutritional Components and Delivery Methods
6. Cell Line Identification
7. Detection of Microbial and Viral Contamination in Cell Lines
Part 2 Culture of Special Cell Types: Stem Cells
8. Neural Crest Stem Cells
9. Primary Animal Skeletal Stem Cells: Methods for Isolating and Analyzing Bone Marrow Stromal Cells BMSCs from Primary Mouse and Human Bone Marrow
10. Establishing Embryonic Stem Cell Lines from Adult Somatic Cells by Nuclear Transfer
11. Isolation and In Vitro Proliferation of T Cells
12. Generation of Human and Mouse Dendritic Cells
Part 3 Culture of Special Cell Types: Hematopoietic Cells, Mesenchymal Cells and Epithelial Cells
13. Basic In Vitro Clonal Culture of Hematopoietic Cells in Semi-Solid Agar Culture
14. Human Skeletal Muscle Cells
15. Growth of Canine Kidney Cells and Their Application in Epithelial Cell Biology Research
16. Culture of Human Epidermal Keratinocytes and Retroviral Infection
17. Three-Dimensional Culture of Normal and Malignant Human Mammary Epithelial Cells to Maintain In Vivo-Like Architecture
18. Primary Culture of Drosophila Embryonic Cells
19. Laboratory Culture of Nematodes and Other Non-Symbiotic Nematodes
Part 4 Somatic Cell Differentiation and Reprogramming
Part 5 Immortality
Part 6 Somatic Cell Hybridization
Part 7 Cell Separation Techniques
Part 8 Cell Cycle Analysis
Part 9 Cytotoxicity and Cell Growth Assays
Part 10 Apoptosis
Part 11 Cell Transformation, Tumorigenicity, Invasion and Wound Healing Assays
Part 12 Electrophysiological Methods
Part 13 Organ Culture
Part 14 Virus Growth and Purification
C Antibodies
Part 15 Production and Purification of Antibodies
D Immunocytochemistry
Part 16 Immunofluorescence
E Appendix
Volume 2Preface
A Organelles and Cell Structure
Part 1 Isolation: Plasma Membrane, Organelles, Cell Structures
1 Detergent-Resistant Membranes and Cholesterol Removal
2 Isolation and Subfractionation of Plasma Membranes, Purification of Caveolae from Lipid Rafts
3 Immunoisolation of Organelles Using Magnetic Solid-Phase Supports
4 Isolation of the Golgi Complex from Rat Liver
5 Isolation of Structural Domains of Rough and Smooth Membranes of Rat Liver Endoplasmic Reticulum
6 Purification of COPI Vesicles
7 Purification of Clathrin-Coated Vesicles from Midbrain, Liver and Adrenal Gland
8 Isolation of Phagosomes Containing Latex Beads and Mycobacteria
9 Isolation of Peroxisomes
10 Isolation of Pure Mitochondria from Mammalian Tissues and Cultured Cells
11 Subcellular Fractionation and Isolation of Dense-Core Secretory Granules from Neuroendocrine Cell Lines Using [S] Sulfate Metabolic Labeling
12 Preparation of Synaptic Vesicles from Mammalian Brain
13 Preparation of Proteasomes
14 Preparation of Cilia from Human Tracheal Epithelial Cells
15 Isolation of Nucleoli
16 Purification of Spliceosomes Containing Intermediates
17 Isolation of Cajal Bodies
18 Replication Clusters: Labeling Strategies for Chromosome Building and Chromatin Dynamics Analysis
19 Chromosome Isolation for Flow Analysis and Sorting
Part 2 Live Staining of Cells/Organelles
20 Live Staining of Cells with Fluorescent Lipids
21 Labeling Endocytic Vesicles with Fluorescent Probes to Study Fluid-Phase Endocytosis
Part 3 Protein Purification
22 Preparation of Porcine Brain Tubulin
23 Purification of Smooth Muscle Actin
……
B Assay Methods
Part 4 Endocytic and Exocytic Pathways
Part 5 Membranes
Part 6 Pure Materials
Part 7 Nuclear Transport
Part 8 Chromatin Assembly
Part 9 Signal Transduction Assays
Part 10 Detection and Models of Motility In Vitro and In Vivo
Part 11 Mechanical Stress in Single Cells
C AppendixVolume 3
Preface
A Imaging Techniques
Part 1 Optical Microscopy
1. Fluorescence Microscopy
2. Total Internal Reflection Fluorescence Microscopy
3. Frequency Limitations in Microscopy and Appropriate Sampling
4. Optical Tweezers: Applications in Dynein Research
Part 2 Digital Image Microscopy
5. How to Acquire Electronic Images in Optical Microscopic Observation of Biological Specimens - Introduction
6. Image Contrast Enhancement Microscopy
Part 3 Confocal Microscopy of Living and Fixed Cells
7. Spinning Disk Confocal Microscopy of Living Cells
8. Confocal Microscopy of Drosophila Embryos
9. Application of UV Laser Microbeams in Drosophila Embryo Dissection
Part 4 Fluorescence Microscopy of Living Cells
10. Introduction to Live-Cell Fluorescence Imaging: Item-by-Item Annotations
11. Cytoskeletal Proteins
12. Systematic Cellular Localization of Novel Proteins
13. Single-Molecule Imaging in Living Cells by Total Internal Reflection Fluorescence Microscopy
14. Live-Cell Fluorescence Speckle Microscopy of Actin Cytoskeleton Dynamics and Drug Perfusion Interference
15. Imaging Fluorescence Resonance Energy Transfer Between Green Fluorescent Protein Variants in Living Cells
Part 5 Application of Fluorescent Dyes to Study Physical Parameters in Cells
16. Determination of pH in Living Cell Endosomes Using Dual-Excitation Fluorescence Imaging
17. Genome-Wide Screening of Protein Localization in Fission Yeast Cells
18. Large-Scale Protein Localization in Yeast
Part 6 Digital Imaging Production, Analysis, Storage and Presentation
19. Dehazing: Reconstructing Images with Deconvolution Techniques
20. Overview of Techniques in Biological Imaging Analysis
21. Imaging Data in Biological Imaging Databases, A Biologist's Imaging Database
B Electron Microscopy
Part 7 Specimen Preparation Techniques
22. Fixation and Embedding of Cells and Tissues for Transmission Electron Microscopy
23. Negative Staining Techniques
24. Glycerol Spray/Low-Angle Rotary Metal Shadowing
Part 8 Cryotechniques
25. Rapid Freezing of Biological Specimens for Freeze-Fracturing and Deep Etching
26. Freeze-Fracturing and Freeze-Etching
Part 9 Electron Microscopy of the Cytoskeleton
27. Electron Microscopy of Extracted Cytoskeletons: Negative Staining, Cryo-Electron Microscopy, and Correlation with Light Microscopy
28. Correlation of Light and Electron Microscopy of the Cytoskeleton
Part 10 Immunoelectron Microscopy
29. Immunoelectron Microscopy with Lowicryl Resin
30. Immunocytochemistry of Ultrathin Frozen and Plastic Sections
31. Direct Immunogold Labeling of Protein Complex Components
C Scanning Probe and Scanning Electron Microscopy
Part 11 Scanning Probe and Scanning Electron Microscopy
32. Atomic Force Microscopy in Biological Research
33. Field Emission Scanning Electron Microscopy and Observation of the Cell Interior
D Microdissection
Part 12 Microdissection of Tissues and Chromosomes
34. Laser Capture Microdissection
35. Chromosome Microdissection Using Conventional Methods
36. Micromanipulation of Chromosomes and the Mitotic Spindle Using Laser Microsurgery (Laser Scissors) and Laser-Generated Optical Forces (Laser Tweezers)
E Tissue Arrays
Part 13 Tissue Arrays
37. Tissue Microarrays
F Cytogenetics and In Situ Hybridization
Part 14 Cytogenetics
38. Basic Cytogenetic Techniques: Culture, Slide Preparation and G-Banding
39. A Common and Reliable Method for Obtaining High-Yield Metaphase Chromosome Specimens from Adherently Growing Cell Lines: Rapid Confirmation of Cellular Chromosome Composition
Part 15 In Situ Hybridization
40. Mapping Cloned DNA on Metaphase Chromosomes by Fluorescence In Situ Hybridization
41. Breakpoint Mapping Using Human Genome Project Resources
42. Fine Mapping of Gene Order Using Extended Chromatin Methods
43. In Situ Hybridization for mRNA Species in Cultured Cells
44. Simultaneous Detection of DNA, RNA and Protein by In Situ Hybridization
45. Fluorescence Observation of Genome Structure and DNA Replication at the Single-Molecule Level
G Genomics
Part 16 Genomics
46. Genomic DNA Microarrays for Comparative Genomic Hybridization
47. Single Nucleotide Polymorphism Genotyping Using Tag Arrays and Microsequencing
48. Single Nucleotide Polymorphism Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
49. Single Nucleotide Polymorphism Analysis Using ZipCode Tag Microsphere Technology
50. PCR Amplification Methods for Preserving Quantitative Differences Between Two Complex Genomes
H Methods for Transgenesis, Gene Knockout and Gene Knockdown
Part 17 Methods for Transgenesis, Gene Knockout and Gene Knockdown
51. Production of Transgenic Mice by Pronuclear Microinjection
52. Gene Targeting by Homologous Recombination in Embryonic Stem Cells
53. Conditional Gene Knockout: The Cre-lox System
54. RNAi-Mediated Gene Silencing in Mammalian Cells
55. Antisense Oligonucleotides
Volume 4Table of Contents Other Tables of Contents
Contributors
Preface
A Transfer of Macromolecules
Part 1 Proteins
Part 2 Genes
Part 3 Somatic Cell Nuclear Transfer
B Expression Systems
Part 4 Expression Systems
C Gene Expression Models
Part 5 Differential Gene Expression
D Proteins
Part 6 Protein Identification and Analysis
Part 7 Specimen Fractionation for Proteomics
Part 8 Gel Electrophoresis
Part 9 Detection of Proteins in Gels
Part 10 Gel Pattern Analysis of Post-Translationally Modified Proteins
Part 11 Protein/Protein and Protein/Small Molecule Interactions
Part 12 Omics of Functional Proteins
Part 13 Protein/DNA Interactions
Part 14 Protein Degradation
Part 15 Mass Spectrometry: Protein Identification and Interactions
E
Part 16 Appendix
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